Overview

  • Product name
  • Description
    Rabbit polyclonal to JIP1
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic non-phosphopeptide derived from human JIP1 around the phosphorylation site of threonine 103 (G-D-TP-P-G).

  • Positive control
    • Human brain tissue. Extracts from HUVEC cells. 3T3 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab78948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 78 kDa.
ELISA 1/5000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

Target

  • Relevance
    Function: The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Required for JNK activation in response to excitotoxic stress. Cytoplasmic MAPK8IP1 causes inhibition of JNK-regulated activity by retaining JNK in the cytoplasm and inhibiting JNK phosphorylation of c-Jun. May also participate in ApoER2-specific reelin signaling. Directly, or indirectly, regulates GLUT2 gene expression and beta-cell function. Appears to have a role in cell signaling in mature and developing nerve terminals. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins (By similarity). Functions as an anti-apoptotic protein and whose level seems to influence the beta-cell death or survival response. Tissue specificity: Highly expressed in brain. Expressed in neurons, localizing to neurite tips in differentiating cells. Also expressed in the pancreas, testis and prostate. Low levels in heart, ovary and small intestine. Decreased levels in pancreatic beta cells sensitize cells to IL-1-beta-induced apoptosis. Disease: Diabetes mellitus, non-insulin-dependent Similarity: Belongs to the JIP scaffold family. Contains 1 PID domain. Contains 1 SH3 domain. Domain: The destruction boxes (D-box) may act as recognition signals for degradation via the ubiquitin-proteasome pathway. A minimal inhibitory domain prevents pancreatic beta cell apoptosis in vitro, and prevents activation of c-jun by MAPK8, MAPK9 and MAPK10. The SH3 domain mediates homodimerization. PTM: Phosphorylated by MAPK8, MAPK9 and MAPK10. Phosphorylation on Thr-103 is also necessary for the dissociation and activation of MAP3K12. Phosphorylated by isoform 1 and isoform 2 of VRK2. Hyperphosphorylated during mitosis following activation of stress-activated and MAP kinases. Ubiquitinated. Two preliminary events are required to prime for ubiquitination; phosphorylation and an increased in intracellular calcium concentration. Then, the calcium influx initiates ubiquitination and degradation by the ubiquitin-proteasome pathway.
  • Cellular localization
    Cytoplasmic. Accumulates in cell surface projections. Under certain stress conditions, translocates to the perinuclear region of neurons. In insulin-secreting cells, detected in both the cytoplasm and nucleus
  • Database links
  • Alternative names
    • C jun amino terminal kinase interacting protein 1 antibody
    • C-jun-amino-terminal kinase-interacting protein 1 antibody
    • IB 1 antibody
    • IB-1 antibody
    • IB1 antibody
    • Islet brain 1 antibody
    • Islet-brain 1 antibody
    • JIP 1 antibody
    • JIP-1 antibody
    • JIP1_HUMAN antibody
    • JNK interacting protein 1 antibody
    • JNK MAP kinase scaffold protein 1 antibody
    • JNK-interacting protein 1 antibody
    • MAPK8IP 1 antibody
    • MAPK8IP1 antibody
    • MAPK8IP1 mitogen-activated protein kinase 8 interacting protein 1 antibody
    • Mitogen activated protein kinase 8 interacting protein 1 antibody
    • Mitogen-activated protein kinase 8-interacting protein 1 antibody
    • PRKM8 antibody
    • PRKM8 interacting protein antibody
    • PRKM8IP antibody
    see all

Anti-JIP1 antibody images

  • All lanes : Anti-JIP1 antibody (ab78948) at 1/500 dilution

    Lane 1 : Extracts from HUVEC cells
    Lane 2 : Extracts from HUVEC cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 78 kDa
    Observed band size : 78 kDa
  • Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab78948 at 1/50 dilution in the absence (left panel) or presence (right panel) of immunising peptide.
  • Immunofluorescence analysis of 3T3 cells using ab78948 at 1/500 dilution in the absence (left panel) or presence (right panel) of immunising peptide.

References for Anti-JIP1 antibody (ab78948)

ab78948 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab78948.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up