Overview

  • Product name
  • Description
    Rabbit polyclonal to JIP2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 529-804 of Human JIP2 (UniProt ID: Q13387).

  • Positive control
    • 293T, A431, H1299, HeLa, HepG2, Molt4, Raji and mouse brain whole cell lysates; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab154090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 88 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. JIP2 inhibits IL1 beta-induced apoptosis in insulin-secreting cells. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins.
  • Tissue specificity
    Expressed mainly in the brain and pancreas, including insulin-secreting cells. In the nervous system, more abundantly expressed in the cerebellum, pituitary gland, occipital lobe and the amygdala. Also expressed in fetal brain. Very low levels found in uterus, ovary, prostate, colon, testis, adrenal gland, thyroid gland and salivary gland.
  • Sequence similarities
    Belongs to the JIP scaffold family.
    Contains 1 PID domain.
    Contains 1 SH3 domain.
  • Cellular localization
    Cytoplasm. Accumulates in cell surface projections.
  • Information by UniProt
  • Database links
  • Alternative names
    • C jun amino terminal kinase interacting protein 2 antibody
    • C-jun-amino-terminal kinase-interacting protein 2 antibody
    • Homologous to mouse JIP 1 antibody
    • IB 2 antibody
    • IB-2 antibody
    • IB2 antibody
    • Islet brain 2 antibody
    • Islet-brain-2 antibody
    • JIP 2 antibody
    • JIP-2 antibody
    • JIP2 antibody
    • JIP2_HUMAN antibody
    • JNK interacting protein 2 antibody
    • JNK MAP kinase scaffold protein 2 antibody
    • JNK MAP kinase scaffold protein JIP2 antibody
    • JNK-interacting protein 2 antibody
    • MAPK8IP2 antibody
    • Mitogen activated protein kinase 8 interacting protein 2 antibody
    • Mitogen-activated protein kinase 8-interacting protein 2 antibody
    • PRKM8 interacting protein like antibody
    • PRKM8IPL antibody
    see all

Images

  • Anti-JIP2 antibody (ab154090) at 1/1000 dilution + Molt4 whole cell lysate at 30 µg

    Predicted band size: 88 kDa



    7.5% SDS PAGE
  • Anti-JIP2 antibody (ab154090) at 1/1000 dilution + mouse brain whole cell lysate at 50 µg

    Predicted band size: 88 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of methanol-fixed HeLa cells labeling JIP2 with ab154090 at 1/200 dilution. The image in the lower panel is costained with Hoechst 33342.

References

This product has been referenced in:
  • Haidar M  et al. miR-126-5p by direct targeting of JNK-interacting protein-2 (JIP-2) plays a key role in Theileria-infected macrophage virulence. PLoS Pathog 14:e1006942 (2018). Read more (PubMed: 29570727) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab154090.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up