Anti-JIP3 antibody (ab4545)

Overview

  • Product nameAnti-JIP3 antibody
    See all JIP3 primary antibodies
  • Description
    Goat polyclonal to JIP3
  • SpecificityThis antibody is expected to recognise both human isoforms of this protein.
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Cow, Human
  • Immunogen

    Synthetic peptide:

    MEIQMDEGGGVV

    , corresponding to N terminal amino acids 2-13 of Human JIP3.

  • General notesGenBank Accession Number – NP_055948; NP_203750. LocusLink ID - 23162 (human); 30957 (mouse). Gene Ontology terms - vesicle-mediated transport; kinesin complex; cytoplasm; kinesin binding; MAP-kinase scaffold activity; protein kinase binding; regulation of JNK cascade.

Properties

Applications

Our Abpromise guarantee covers the use of ab4545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionThe JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. May function as a regulator of vesicle transport, through interations with the JNK-signaling components and motor proteins.
  • Sequence similaritiesBelongs to the JIP scaffold family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-jun-amino-terminal kinase-interacting protein 3 antibody
    • FLJ00027 antibody
    • Homolog of Drosophila Sunday driver 2 antibody
    • JIP-3 antibody
    • JIP3 antibody
    • JIP3_HUMAN antibody
    • JNK / Stress Activated Protein Kinase Associated Protein 1 antibody
    • JNK Interacting Protein 3 antibody
    • JNK MAP kinase scaffold protein 3 antibody
    • JNK-interacting protein 3 antibody
    • JNK/SAPK associated protein 1 antibody
    • JSAP1 antibody
    • KIAA1066 antibody
    • MAPK8IP3 antibody
    • Mitogen Activated Protein kinase 8 Interacting Protein 3 antibody
    • Mitogen-activated protein kinase 8-interacting protein 3 antibody
    • Syd antibody
    • SYD2 antibody
    see all

Anti-JIP3 antibody images

  • ab4545 (4µg/ml) staining of paraffin embedded Mouse Brain. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. Shows axonal staining in various regions

References for Anti-JIP3 antibody (ab4545)

ab4545 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rabbit Tissue lysate - whole (heart)
Loading amount 60 µg
Specification heart
Gel Running Conditions Non-reduced Denaturing (7.5)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Feb 15 2012

Thank you for your email. To ship to Canada, the cost is 60 USD (United States Dollar). If you have any more questions, please let me know.

Thank you for your email. We have not yet received feedback regarding this antibody so it is still possible for you to obtain the $300 Conference Travel Award. A discount is not available for this antibody. If you have any more questions, just let us k...

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Thank you for your enquiry. Ab4545 is a Fast-Track antibody; Fast-Track antibodies are immunogen affinity purified and are shown by ELISA to have high titre values against their immunogen. We are currently testing them for use in Western blotting. At t...

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