Anti-JIP3 antibody (ab4545)

Abreviews (1)Q&A (3)
Customer reviews and FAQs

Overview

  • Product name
    Anti-JIP3 antibody
    See all JIP3 primary antibodies
  • Description
    Goat polyclonal to JIP3
  • Specificity
    This antibody is expected to recognise both human isoforms of this protein.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Cow, Human
  • Immunogen

    Synthetic peptide:

    MEIQMDEGGGVV

    , corresponding to N terminal amino acids 2-13 of Human JIP3.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes
    GenBank Accession Number – NP_055948; NP_203750. LocusLink ID - 23162 (human); 30957 (mouse). Gene Ontology terms - vesicle-mediated transport; kinesin complex; cytoplasm; kinesin binding; MAP-kinase scaffold activity; protein kinase binding; regulation of JNK cascade.

Properties

Applications

Our Abpromise guarantee covers the use of ab4545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. May function as a regulator of vesicle transport, through interations with the JNK-signaling components and motor proteins.
  • Sequence similarities
    Belongs to the JIP scaffold family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • C-jun-amino-terminal kinase-interacting protein 3 antibody
    • FLJ00027 antibody
    • Homolog of Drosophila Sunday driver 2 antibody
    • JIP-3 antibody
    • JIP3 antibody
    • JIP3_HUMAN antibody
    • JNK / Stress Activated Protein Kinase Associated Protein 1 antibody
    • JNK Interacting Protein 3 antibody
    • JNK MAP kinase scaffold protein 3 antibody
    • JNK-interacting protein 3 antibody
    • JNK/SAPK associated protein 1 antibody
    • JSAP1 antibody
    • KIAA1066 antibody
    • MAPK8IP3 antibody
    • Mitogen Activated Protein kinase 8 Interacting Protein 3 antibody
    • Mitogen-activated protein kinase 8-interacting protein 3 antibody
    • Syd antibody
    • SYD2 antibody
    see all

Anti-JIP3 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JIP3 antibody (ab4545)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JIP3 antibody (ab4545)
    ab4545 (4µg/ml) staining of paraffin embedded Mouse Brain. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. Shows axonal staining in various regions

References for Anti-JIP3 antibody (ab4545)

ab4545 has not yet been referenced specifically in any publications.

Publishing research using ab4545? Please let us know so that we can cite the reference in this datasheet.

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Western blot abreview for Anti-JIP3 antibody

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Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rabbit Tissue lysate - whole (heart)
Loading amount
60 µg
Specification
heart
Gel Running Conditions
Non-reduced Denaturing (7.5)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Verified customer

Submitted Feb 15 2012

Hi Jennifer, I am wondering that Fright/packing fee $60 is USD or CaD? This price show in website. I am a little confused.

Thank you for your email. To ship to Canada, the cost is 60 USD (United States Dollar). If you have any more questions, please let me know.

Thank you very much for your information I want to oder Jip3 antibody.It is impossible for me to get the award $300 of Conference Travel Money to the first researcher who sends you positive feedback. I am just wondering if I get any discount of this p...

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Thank you for your email. We have not yet received feedback regarding this antibody so it is still possible for you to obtain the $300 Conference Travel Award. A discount is not available for this antibody. If you have any more questions, just let us k...

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I am a posdoctor in department of Biochemistry and Medical Genetics o the university of Manitoba at Canada. I want t order JIP3 antibody. I have never used this antibody before. So, Can you send me some detail information about this antibody? For examp...

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Thank you for your enquiry. Ab4545 is a Fast-Track antibody; Fast-Track antibodies are immunogen affinity purified and are shown by ELISA to have high titre values against their immunogen. We are currently testing them for use in Western blotting. At t...

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