Overview

  • Product name
  • Description
    Rabbit polyclonal to JLP
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Opossum, Orangutan, Elephant
  • Immunogen

    Immunogen was a synthetic peptide, which represented a portion of human JLP encoded within exon 1.

  • Positive control
    • HeLa nuclear extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab12331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 156.8 kDa.
IP Use a concentration of 2 - 5 µg/ml.

Target

  • Relevance
    JLP is a member of the JNK-interacting protein (JIP) group of scaffold proteins which selectively mediate JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module.
  • Cellular localization
    Cytoplasm. Cytoplasm, perinuclear region. Note: Perinuclear distribution in response to stress signals such as UV radiation.
  • Database links
  • Alternative names
    • C jun amino terminal kinase interacting protein 4 antibody
    • c Jun NH2 terminal kinase associated leucine zipper protein antibody
    • FLJ13450 antibody
    • FLJ14006 antibody
    • FLJ34602 antibody
    • HLC 4 antibody
    • HLC 4 protein antibody
    • HLC 6 antibody
    • HLC4 antibody
    • HLC4 protein antibody
    • HLC6 antibody
    • HSS antibody
    • Human lung cancer protein 6 antibody
    • JIP 4 antibody
    • JIP4 antibody
    • JNK associated leucine zipper protein 1 antibody
    • JNK associated leucine zipper protein antibody
    • JNK interacting protein 4 antibody
    • JNK interacting protein antibody
    • JNK/SAPK associated protein antibody
    • KIAA0516 antibody
    • Lung cancer oncogene 4 antibody
    • MAPK8IP4 antibody
    • Max binding protein antibody
    • MGC117291 antibody
    • MGC14967 antibody
    • MGC74461 antibody
    • Mitogen activated protein kinase 8 interacting protein 4 antibody
    • PHET antibody
    • PIG 6 antibody
    • PIG6 antibody
    • Proliferation inducing gene 6 antibody
    • Protein highly expressed in testis antibody
    • SPAG 9 antibody
    • SPAG9 antibody
    • Sperm associated antigen 9 antibody
    • Sperm specific protein antibody
    • Sperm surface protein antibody
    • Sunday driver (drosophila) homolog antibody
    • Sunday driver 1 antibody
    • SYD 1 antibody
    • SYD1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis (left) and testicular seminoma (right) tissues labelling JLP with ab12331 at 1/5000 (0.2µg/ml) and 1/1000 (1µg/ml). Detection: DAB.
  • ICC/IF image of ab12331 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12331, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-JLP antibody (ab12331) at 0.2 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 156.8 kDa


    Exposure time : 3 minutes
  • All lanes : Anti-JLP antibody (ab12331) at 1 µg/ml

    Lane 1 : NIH3T3 whole cell lysate
    Lane 2 : TCMK-1 whole cell lysate
    Lane 3 : 4T1 whole cell lysate
    Lane 4 : CT26.WT whole cell lysate
    Lane 5 : C6 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique

    Predicted band size : 156.8 kDa


    Exposure time : 3 minutes
  • Immunoprecipitation analysis of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded). ab12331 was used for immunoprecipitation at 3 µg/mg lysate and used for western blot at 1 µg/ml. Lanes 1 and 2 - ab12331 IP, Lane 3 - control IgG.

References

This product has been referenced in:
  • Zhao XC  et al. Isolating Sperm from Cell Mixtures Using Magnetic Beads Coupled with an Anti-PH-20 Antibody for Forensic DNA Analysis. PLoS One 11:e0159401 (2016). Read more (PubMed: 27442128) »
  • Xiao C  et al. SPAG9 is overexpressed in osteosarcoma, and regulates cell proliferation and invasion through regulation of JunD. Oncol Lett 12:2674-2679 (2016). IHC ; Human . Read more (PubMed: 27698841) »

See all 9 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (muscle cell)
Loading amount
30 µg
Specification
muscle cell
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Bok-Geon Kim

Verified customer

Submitted Jul 08 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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