Overview

  • Product nameAnti-JLP antibody
    See all JLP primary antibodies
  • Description
    Rabbit polyclonal to JLP
  • Tested applicationsSuitable for: IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Baboon, Rhesus monkey, Orangutan
  • Immunogen

    Immunogen was a synthetic peptide, which represented a portion of human JLP encoded within exons 21 and 22.

  • Positive control
    • HeLa nuclear extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab12332 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 156.8 kDa.
IP Use at 6 µg/mg of lysate.

Target

  • RelevanceJLP is a member of the JNK-interacting protein (JIP) group of scaffold proteins which selectively mediate JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module.
  • Cellular localizationCytoplasm. Cytoplasm, perinuclear region. Note: Perinuclear distribution in response to stress signals such as UV radiation.
  • Database links
  • Alternative names
    • C jun amino terminal kinase interacting protein 4 antibody
    • c Jun NH2 terminal kinase associated leucine zipper protein antibody
    • FLJ13450 antibody
    • FLJ14006 antibody
    • FLJ34602 antibody
    • HLC 4 antibody
    • HLC 4 protein antibody
    • HLC 6 antibody
    • HLC4 antibody
    • HLC4 protein antibody
    • HLC6 antibody
    • HSS antibody
    • Human lung cancer protein 6 antibody
    • JIP 4 antibody
    • JIP4 antibody
    • JNK associated leucine zipper protein 1 antibody
    • JNK associated leucine zipper protein antibody
    • JNK interacting protein 4 antibody
    • JNK interacting protein antibody
    • JNK/SAPK associated protein antibody
    • KIAA0516 antibody
    • Lung cancer oncogene 4 antibody
    • MAPK8IP4 antibody
    • Max binding protein antibody
    • MGC117291 antibody
    • MGC14967 antibody
    • MGC74461 antibody
    • Mitogen activated protein kinase 8 interacting protein 4 antibody
    • PHET antibody
    • PIG 6 antibody
    • PIG6 antibody
    • Proliferation inducing gene 6 antibody
    • Protein highly expressed in testis antibody
    • SPAG 9 antibody
    • SPAG9 antibody
    • Sperm associated antigen 9 antibody
    • Sperm specific protein antibody
    • Sperm surface protein antibody
    • Sunday driver (drosophila) homolog antibody
    • Sunday driver 1 antibody
    • SYD 1 antibody
    • SYD1 antibody
    see all

Anti-JLP antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling JLP with ab12332 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-JLP antibody (ab12332) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 156.8 kDa


    Exposure time : 30 seconds
  • All lanes : Anti-JLP antibody (ab12332) at 0.1 µg/ml

    Lane 1 : TCKM whole cell lysate at 50 µg
    Lane 2 : Renca whole cell lysate at 15 µg
    Lane 3 : CT26 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 156.8 kDa


    Exposure time : 10 seconds


  • Predicted band size : 156.8 kDa

    Samples: Nuclear extract (NE, 20 µg) from HeLa cells. Antibody: Affinity purified rabbit anti-JLP antibody ab12332 used at 0.3 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

     

    Samples: Nuclear extract (NE, 20 µg) from HeLa cells. Antibody: Affinity purified rabbit anti-JLP antibody ab12332 used at 0.3 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
  • Immunoprecipitation analaysis of HeLa whole cell lysate (1 mg for IP; 20% of IP loaded). ab12332 was used for immunoprecipitation at 6 µg/mg lysate (lanes 1 and 2). Lane 3 - control IgG. For western blot ab12332 was used at 1 µg/ml.

References for Anti-JLP antibody (ab12332)

This product has been referenced in:
  • Blüher M  et al. Activated Ask1-MKK4-p38MAPK/JNK stress signaling pathway in human omental fat tissue may link macrophage infiltration to whole-body Insulin sensitivity. J Clin Endocrinol Metab 94:2507-15 (2009). Read more (PubMed: 19351724) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"