Anti-JM4/PRAF2 antibody (ab53113)
Key features and details
- Rabbit polyclonal to JM4/PRAF2
- Suitable for: IHC-P, ELISA, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-JM4/PRAF2 antibody
See all JM4/PRAF2 primary antibodies -
Description
Rabbit polyclonal to JM4/PRAF2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ELISA, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53113 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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WB | (1) |
1/300 - 1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
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Notes |
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IHC-P
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
WB
1/300 - 1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa). |
Target
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Function
May be involved in ER/Golgi transport and vesicular traffic. Plays a proapoptic role in cerulenin-induced neuroblastoma apoptosis. -
Tissue specificity
Strong expression in the brain, small intestine, lung, spleen, and pancreas as well as in tumor tissues of the breast, colon, lung and ovary, with a weaker expression in normal tissues of the same patient. High expression in neuroblastic tumors. Strongly expressed in Purkinje cells and more moderately in cells of the molecular and the granular layers in the cerebellum. Detected in neuronal cells, but not in non-neuronal cells in the cerebral cortex, hippocampus, and lateral ventricles. -
Sequence similarities
Belongs to the PRA1 family. -
Cellular localization
Endosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 11230 Human
- Entrez Gene: 54637 Mouse
- Entrez Gene: 367743 Rat
- SwissProt: O60831 Human
- SwissProt: Q9JIG8 Mouse
- Unigene: 29595 Human
- Unigene: 28850 Mouse
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Alternative names
- Jena Muenchen 4 antibody
- JM 4 antibody
- PRA1 domain family member 2 antibody
see all
Images
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All lanes : Anti-JM4/PRAF2 antibody (ab53113) at 1/300 dilution
Lane 1 : Mouse spleen whole tissue lysate
Lane 2 : Mouse braint whole tissue lysate
Lane 3 : Mouse heart whole tissue lysate
Lane 4 : Mouse kidney whole tissue lysate
Lane 5 : Mouse liver whole tissue lysate
Lane 6 : Mouse embryonic fibroblasts lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : HRP-conjugated Goat anti-rabbit at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 19 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa (possible non-specific binding)
Exposure time: 20 seconds
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All lanes : Anti-JM4/PRAF2 antibody (ab53113) at 1/300 dilution
Lane 1 : LOVO cell extract
Lane 2 : LOVO cell extract with immunising peptide
Predicted band size: 19 kDa
Observed band size: 19 kDa -
ab53113 at 1/50 dilution staining JM4/PRAF2 in human brain tissue by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab53113 has been referenced in 2 publications.
- He W et al. Mutual regulation of JAG2 and PRAF2 promotes migration and invasion of colorectal cancer cells uncoupled from epithelial-mesenchymal transition. Cancer Cell Int 19:160 (2019). PubMed: 31198409
- Chiu HC et al. High-throughput quantitative proteomic analysis of dengue virus type 2 infected A549 cells. PLoS One 9:e93305 (2014). WB ; Human . PubMed: 24671231