• Product name
  • Description
    Rabbit polyclonal to JM4
  • Specificity
    ab53113 detects endogenous levels of total JM4 protein.
  • Tested applications
    Suitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    A synthetic peptide derived from human JM4.

  • Positive control
    • Human brain tissue and extracts from LOVO cells.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab53113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ELISA 1/40000.
WB 1/300 - 1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).


  • Function
    May be involved in ER/Golgi transport and vesicular traffic. Plays a proapoptic role in cerulenin-induced neuroblastoma apoptosis.
  • Tissue specificity
    Strong expression in the brain, small intestine, lung, spleen, and pancreas as well as in tumor tissues of the breast, colon, lung and ovary, with a weaker expression in normal tissues of the same patient. High expression in neuroblastic tumors. Strongly expressed in Purkinje cells and more moderately in cells of the molecular and the granular layers in the cerebellum. Detected in neuronal cells, but not in non-neuronal cells in the cerebral cortex, hippocampus, and lateral ventricles.
  • Sequence similarities
    Belongs to the PRA1 family.
  • Cellular localization
    Endosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Jena Muenchen 4 antibody
    • JM 4 antibody
    • PRA1 domain family member 2 antibody
    • PRA1 family protein 2 antibody
    • PRAF2 antibody
    • PRAF2_HUMAN antibody
    see all

Anti-JM4 antibody images

  • All lanes : Anti-JM4 antibody (ab53113) at 1/300 dilution

    Lane 1 : Mouse spleen whole tissue lysate
    Lane 2 : Mouse braint whole tissue lysate
    Lane 3 : Mouse heart whole tissue lysate
    Lane 4 : Mouse kidney whole tissue lysate
    Lane 5 : Mouse liver whole tissue lysate
    Lane 6 : Mouse embryonic fibroblasts lysate

    Lysates/proteins at 40 µg per lane.

    HRP-conjugated Goat anti-rabbit at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 19 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa (possible non-specific binding).

    Exposure time : 20 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

  • All lanes : Anti-JM4 antibody (ab53113) at 1/300 dilution

    Lane 1 : LOVO cell extract
    Lane 2 : LOVO cell extract with immunising peptide

    Predicted band size : 19 kDa
    Observed band size : 19 kDa
  • ab53113 at 1/50 dilution staining JM4 in human brain tissue by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.ab53113 at 1/50 dilution staining JM4 in human brain tissue by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.

References for Anti-JM4 antibody (ab53113)

This product has been referenced in:
  • Chiu HC  et al. High-throughput quantitative proteomic analysis of dengue virus type 2 infected A549 cells. PLoS One 9:e93305 (2014). WB ; Human . Read more (PubMed: 24671231) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (12,5)
Mouse Tissue lysate - whole (Spleen, Liver, Heart, Brain, kidney)
Spleen, Liver, Heart, Brain, kidney
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 2%

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Verified customer

Submitted Sep 16 2013


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