Overview

  • Product name
  • Description
    Rabbit polyclonal to JMJD4
  • Specificity
    ab106458 does not recognize other members of the JMJD family.
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    A 17 amino acid synthetic peptide from near the amino terminus of Human JMJD4 (NP_075383).

  • Positive control
    • WB: Human spleen tissue lysate IHC-P: Rat spleen lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab106458 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 52 kDa.

Target

Images

  • Immunofluorescence of JMJD4 in rat spleen tissue with ab106458 at 5 ug/mL.

  • All lanes : Anti-JMJD4 antibody (ab106458) at 1 µg/ml

    Lane 1 : Human spleen tissue lysate
    Lane 2 : Human spleen tissue lysate with blocking peptide

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 52 kDa
  • ab106458, at 2.5 ug/ml, staining JMJD4 in paraffin-embedded rat spleen tissue.

References

ab106458 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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