Overview

  • Product nameAnti-JMJD5 antibody
    See all JMJD5 primary antibodies
  • Description
    Rabbit polyclonal to JMJD5
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Caenorhabditis elegans, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Human JMJD5. Synthetic peptide derived from residues 350 - 399 of human JMJD5.
    Database link: Q8N371

  • Positive control
    • Fetal liver lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab28883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-JMJD5 antibody images

  • Anti-JMJD5 antibody (ab28883) at 0.5 µg/ml + Human Fetal Liver Lysate

    Secondary
    HRP conjugated anti-Rabbit IgG (suggested dilution 1:50,000 - 100,000)
    Developed using the ECL technique

    Predicted band size : 47 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

References for Anti-JMJD5 antibody (ab28883)

ab28883 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Loading amount 15 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Caenorhabditis elegans Tissue lysate - nuclear (All body)
Specification All body
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Pier Giorgio Amendola

Verified customer

Submitted Jun 24 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"