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Synthetic peptide corresponding to Human JMJD5 aa 288-337 (internal sequence).
Our Abpromise guarantee covers the use of ab83011 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
ChIP analysis using ab83011 (0.5µg/mL) binding JMJD5 in human HCT116 colon cancer cells. Without (- peptide) and with (+ peptide) blocking peptide. Protein binding was detected using real-time PCR.
ab83011 has not yet been referenced specifically in any publications.