Anti-JMJD6 antibody (ab50720)


  • Product nameAnti-JMJD6 antibody
    See all JMJD6 primary antibodies
  • Description
    Rabbit polyclonal to JMJD6
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Zebrafish
  • Immunogen

    A region within synthetic peptide: VLADSVDLQE STGIASDSSS DSSSSSSSSS SDSDSECESG SEGDGTVHRR, corresponding to C terminal amino acids 325-374 of Human JMJD6

  • Positive control
    • Fetal thymus lysate


Associated products


Our Abpromise guarantee covers the use of ab50720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 46 kDa.Can be blocked with JMJD6 peptide (ab110802). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • FunctionDioxygenase that can both act as a histone arginine demethylase and a lysyl-hydroxylase. Acts as a lysyl-hydroxylase that catalyzes 5-hydroxylation on specific lysine residues of target proteins such as U2AF2/U2AF65 and LUC7L2. Acts as a regulator of RNA splicing by mediating 5-hydroxylation of U2AF2/U2AF65, affecting the pre-mRNA splicing activity of U2AF2/U2AF65. In addition to peptidyl-lysine 5-dioxygenase activity, may act as a RNA hydroxylase, as suggested by its ability to bind single strand RNA. Also acts as an arginine demethylase which demethylates histone H3 at 'Arg-2' (H3R2me) and histone H4 at 'Arg-3' (H4R3me), thereby playing a role in histone code. However, histone arginine demethylation may not constitute the primary activity in vivo. Has no histone lysine demethylase activity. Required for differentiation of multiple organs during embryogenesis. Acts as a key regulator of hematopoietic differentiation: required for angiogenic sprouting by regulating the pre-mRNA splicing activity of U2AF2/U2AF65. Seems to be necessary for the regulation of macrophage cytokine responses.
  • Tissue specificityHighly expressed in the heart, skeletal muscle and kidney. Expressed at moderate or low level in brain, placenta, lung, liver, pancreas, spleen, thymus, prostate, testis and ovary. Up-regulated in many patients with chronic pancreatitis. Expressed in nursing thymic epithelial cells.
  • Sequence similaritiesBelongs to the JMJD6 family.
    Contains 1 JmjC domain.
  • DomainThe nuclear localization signal motifs are necessary and sufficient to target it into the nucleus.
  • Cellular localizationNucleus > nucleoplasm. Nucleus > nucleolus. Mainly found throughout the nucleoplasm outside of regions containing heterochromatic DNA, with some localization in nucleolus. During mitosis, excluded from the nucleus and reappears in the telophase of the cell cycle.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptotic cell clearance receptor antibody
    • Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6 antibody
    • Histone arginine demethylase JMJD6 antibody
    • JmjC domain-containing protein 6 antibody
    • JMJD 6 antibody
    • JMJD6 antibody
    • JMJD6_HUMAN antibody
    • Jumonji domain containing 6 antibody
    • Jumonji domain-containing protein 6 antibody
    • KIAA0585 antibody
    • Lysyl-hydroxylase JMJD6 antibody
    • Peptide-lysine 5-dioxygenase JMJD6 antibody
    • Phosphatidylserine receptor antibody
    • Protein PTDSR antibody
    • PSR antibody
    • PTDSR 1 antibody
    • PTDSR antibody
    • PTDSR1 antibody
    see all

Anti-JMJD6 antibody images

  • Anti-JMJD6 antibody (ab50720) at 1.25 µg/ml + Fetal thymus lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 46 kDa

References for Anti-JMJD6 antibody (ab50720)

ab50720 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample Human Cell (293T)
Specification 293T
Permeabilization Yes - Triton
Fixative Formaldehyde

Herr Dr. Alexander Wolf

Verified customer

Submitted Jul 30 2014

Thank you for contacting Abcam.

I am sorry this product has not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a blocking peptide (ab110802) to you with the order number 1021613. Read More

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Recombinant protein (N/A)
Loading amount 0.01 µg
Specification N/A
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 07 2008