• Product nameAnti-JMY antibody
    See all JMY primary antibodies
  • Description
    Rabbit polyclonal to JMY
  • Specificityab69945 detects endogenous levels of total JMY protein.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from an internal sequence of human JMY.

  • Positive control
    • COLO cell extracts.



Our Abpromise guarantee covers the use of ab69945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 112 kDa).
ELISA 1/5000.


  • FunctionActs both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions. In nucleus, acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300. Increases p53/TP53-dependent transcription and apoptosis, suggesting an important role in p53/TP53 stress response such as DNA damage. In cytoplasm, acts as a nucleation-promoting factor for both branched and unbranched actin filaments. Activates the Arp2/3 complex to induce branched actin filament networks. Also catalyzes actin polymerization in the absence of Arp2/3, creating unbranched filaments. Contributes to cell motility by controlling actin dynamics. May promote the rapid formation of a branched actin network by first nucleating new mother filaments and then activating Arp2/3 to branch off these filaments. The p53/TP53-cofactor and actin activator activities are regulated via its subcellular location.
  • Sequence similaritiesBelongs to the JMY family.
    Contains 1 WH2 domain.
  • Post-translational
    Ubiquitinated by MDM2, leading to its subsequent degradation by the proteasome. In case of DNA damage, the interaction with MDM2 is altered, preventing degradation and allowing interaction with p300/EP300 and its function in p53/TP53 stress response.
  • Cellular localizationNucleus. Cytoplasm > cytoskeleton. Localizes to the nucleus in most cell types. Accumulates in nucleus under DNA damage conditions, increasing p53/TP53 transcription response and reducing its influence on cell motility (By similarity). In primary neutrophils, it colocalizes with actin filaments at the leading edge and is excluded from the nucleus. Localization correlates with motility, because it moves from the nucleus to the cytoplasmic compartment when cells are differentiated from nonmotile cells into highly motile neutrophil-like cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ37870 antibody
    • Jmy antibody
    • JMY protein antibody
    • JMY_HUMAN antibody
    • junction mediating and regulatory protein antibody
    • Junction mediating and regulatory protein p53 cofactor antibody
    • Junction-mediating and -regulatory protein antibody
    • MGC163496 antibody
    • OTTHUMP00000161976 antibody
    • WAS protein homology region 2 domain containing 1-like 3 antibody
    • WHDC1L3 antibody
    see all

Anti-JMY antibody images

  • All lanes : Anti-JMY antibody (ab69945) at 1/500 dilution

    Lane 1 : COLO cell exctract
    Lane 2 : COLO cell exctract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 112 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)

References for Anti-JMY antibody (ab69945)

ab69945 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab69945.
Please use the links above to contact us or submit feedback about this product.