Overview

  • Product name
    Anti-JMY antibody - C-terminal
    See all JMY primary antibodies
  • Description
    Rabbit polyclonal to JMY - C-terminal
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human JMY aa 931-980 (C terminal). The exact sequence is proprietary.
    Sequence:

    KGVKLKKVQK DVLRESFTLL PDTDPLTRSI HEALRRIKEA SPESEDEEEA


    Database link: Q8N9B5

  • Positive control
    • Human brain cortex tissue; COLO cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab190212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 111 kDa.

Target

  • Function
    Acts both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions. In nucleus, acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300. Increases p53/TP53-dependent transcription and apoptosis, suggesting an important role in p53/TP53 stress response such as DNA damage. In cytoplasm, acts as a nucleation-promoting factor for both branched and unbranched actin filaments. Activates the Arp2/3 complex to induce branched actin filament networks. Also catalyzes actin polymerization in the absence of Arp2/3, creating unbranched filaments. Contributes to cell motility by controlling actin dynamics. May promote the rapid formation of a branched actin network by first nucleating new mother filaments and then activating Arp2/3 to branch off these filaments. The p53/TP53-cofactor and actin activator activities are regulated via its subcellular location.
  • Sequence similarities
    Belongs to the JMY family.
    Contains 1 WH2 domain.
  • Post-translational
    modifications
    Ubiquitinated by MDM2, leading to its subsequent degradation by the proteasome. In case of DNA damage, the interaction with MDM2 is altered, preventing degradation and allowing interaction with p300/EP300 and its function in p53/TP53 stress response.
  • Cellular localization
    Nucleus. Cytoplasm > cytoskeleton. Localizes to the nucleus in most cell types. Accumulates in nucleus under DNA damage conditions, increasing p53/TP53 transcription response and reducing its influence on cell motility (By similarity). In primary neutrophils, it colocalizes with actin filaments at the leading edge and is excluded from the nucleus. Localization correlates with motility, because it moves from the nucleus to the cytoplasmic compartment when cells are differentiated from nonmotile cells into highly motile neutrophil-like cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ37870 antibody
    • Jmy antibody
    • JMY protein antibody
    • JMY_HUMAN antibody
    • junction mediating and regulatory protein antibody
    • Junction mediating and regulatory protein p53 cofactor antibody
    • Junction-mediating and -regulatory protein antibody
    • MGC163496 antibody
    • OTTHUMP00000161976 antibody
    • WAS protein homology region 2 domain containing 1-like 3 antibody
    • WHDC1L3 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human brain, cortex, tissue labeling JMY using ab190212 at 10 µg/ml.

  • All lanes : Anti-JMY antibody - C-terminal (ab190212) at 1/500 dilution

    Lane 1 : COLO cell lysate
    Lane 2 : COLO cell lysate with Synthesized peptide


    Predicted band size : 111 kDa

References

ab190212 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab190212.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up