JNK 1/2/3 pThr183/Tyr185 + Total PhosphoTracer ELISA Kit (ab119663)

Overview

  • Product nameJNK 1/2/3 pThr183/Tyr185 + Total PhosphoTracer ELISA KitSee all JNK (phospho Thr183/Tyr185) + total JNK kits ...
  • Detection methodFluorescent
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture extracts
  • Assay typeSemi-quantitative
  • Assay time
    2h 0m
  • Assay durationOne step assay
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Product overview

    PhosphoTracer assays use a traditional immuno-sandwich format, but with a major difference both the analyte and the assay reagents are added to the PhosphoTracer assay microplate at the same time. After a short incubation period, unbound assay reagents and analytes are washed away, and immuno-complexes containing both antibodies are detected. The process can take as little as 60 minutes to complete.

    PhosphoTracer kits also allow a higher degree of assay flexibility. In contrast to other ELISA formats, no antibodies are present on the assay microplate itself, so assays for several different targets can be performed in different wells on the same microplate. Simply mix the lysate with your target reagents of choice, using the microplate configuration of your choice.

    A whole new way of performing cellular assays, PhosphoTracer takes the hard work out of running a standard ELISA, while still giving the high quality results expected from a sandwich immunoassay. Fully self-contained kits are supplied in convenient 96-well packs. Simple to use and highly sensitive PhosphoTracer kits are designed to get results, fast.

     

    Abcam's PhosphoTracer JNKassay kits detect endogenous levels of JNK 1/2/3 (GenBank Accessions NP_620637 [JNK1], NP_620707 [JNK2] and NP_620446 [JNK3]) in cellular lysates. The phospho-JNK assays detects JNK only when phosphorylated at Thr183/Tyr185. The total JNK assay detects JNK irrespective of phosphorylation.



    The substrate used with the HRP conjugated detection antibody is a combination of 10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) (wavelength exc/em = 530-540nm / 590-600nm), a highly sensitive and stable substrate for HRP) and ADHP Dilution Buffer (a stabilized H2O2 solution). Learn more about the fluorogenic substrate, ADHP.

  • Notes

    Sensitivity: Phospho-JNK: 50ng/ml (tested with rhJNK1alpha), JNK: 5ng/ml (tested with rhJNK1alpha).

    Range: Phospho-JNK: 50-5000ng/ml (tested with rhJNK1alpha), JNK: 5-5000ng/ml (tested with rhJNK1alpha).

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • FunctionResponds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
    JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications
    Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.
  • Target information above from: UniProt accession P45983 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names
    • c Jun kinase 2
    • c Jun N terminal kinase 2
    • c Jun N terminal kinase 3
    • c-Jun N-terminal kinase 1
    • JNK 55
    • JNK-46
    • JNK1
    • JNK1A2
    • JNK2
    • JNK21B1/2
    • JNK2A
    • JNK2ALPHA
    • JNK2B
    • JNK2BETA
    • JNK3
    • JNK3 alpha protein kinase
    • JNK3A
    • Jun kinase
    • JUN N terminal kinase
    • MAP kinase 10
    • MAP kinase 8
    • MAP kinase 9
    • MAP kinase p49 3F12
    • MAPK 10
    • MAPK 8
    • MAPK 9
    • MAPK10
    • mapk8
    • MAPK9
    • Mitogen activated protein kinase 10
    • Mitogen activated protein kinase 8 isoform JNK1 alpha1
    • Mitogen activated protein kinase 8 isoform JNK1 beta2
    • Mitogen activated protein kinase 9
    • Mitogen-activated protein kinase 8
    • MK08_HUMAN
    • p493F12
    • p54a
    • p54aSAPK
    • p54bSAPK
    • PRKM10
    • PRKM8
    • PRKM9
    • SAPK
    • SAPK1
    • SAPK1a
    • SAPK1b
    • SAPK1c
    • Stress activated protein kinase 1a
    • Stress activated protein kinase 1b
    • Stress activated protein kinase 1c
    • Stress activated protein kinase beta
    • Stress activated protein kinase JNK2
    • Stress activated protein kinase JNK3
    • Stress-activated protein kinase 1
    • Stress-activated protein kinase JNK1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab119663 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent concentration. Phospho-JNK: Tested in HeLa, HEK293, U937, NIH3T3. JNK: Tested in HEK293, HeLa, A431, C2C12, Raw264.7, A549, PC3, NIH3T3, U937.

JNK 1/2/3 pThr183/Tyr185 + Total PhosphoTracer ELISA Kit images

  • Using the JNK 1/2/3 assay kits, a significant stimulation of JNK 1/2/3 phosphorylation at Thr183/Tyr185 is detected in HEK293 cells treated with anisomycin for 30 minutes compared with untreated cells, while no change in total JNK levels is observed.
  • A Dilution series of recombinant active JNK1alpha was prepared by diluting to various concentrations in Lysis Mix containing 0.1% BSA. This dilution series was analyzed using the phospho-JNK assay. Limit of detection of active rhJNK was around 50 ng/mL.
  • PhosphoTracer JNK (pT183/Y185) + total JNK ELISA Kit (ab119663) used in Sandwich ELISA.

Protocols

References for JNK 1/2/3 pThr183/Tyr185 + Total PhosphoTracer ELISA Kit (ab119663)

ab119663 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

We have not particularly tested CSF (cerebrospinal fluid?) with this kit however you can use this kit with any human, mouse or rat samples.

The kit is for qualitative purpose so we may not be able to tell ...

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This kit will detect all three JNK form when they are phosphorylated on pThr183/Tyr185. Moreover, it will also detect the whole of the JNK1/2/3.

The three ELISA kits we have in our catalog differ in the following according to their datas...

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Merci de nous avoir contactés.

Voici la liste des kits

1) pour la détection d'une seule formed'une protéine particulière :

ERK 1/2 (pThr202/Tyr204) : ab119625, http://www.abcam.com/ab119625
Read More

Thank you for contacting Abcam.

I have talked to the lab about using heart tissue with ab119663 and they have told me that we do not have any data with tissue extracts. However, there should be no reason why this should not work.

We...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"