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PhosphoTracer assays use a traditional immuno-sandwich format, but with a major difference both the analyte and the assay reagents are added to the PhosphoTracer assay microplate at the same time. After a short incubation period, unbound assay reagents and analytes are washed away, and immuno-complexes containing both antibodies are detected. The process can take as little as 60 minutes to complete.
PhosphoTracer kits also allow a higher degree of assay flexibility. In contrast to other ELISA formats, no antibodies are present on the assay microplate itself, so assays for several different targets can be performed in different wells on the same microplate. Simply mix the lysate with your target reagents of choice, using the microplate configuration of your choice.
A whole new way of performing cellular assays, PhosphoTracer takes the hard work out of running a standard ELISA, while still giving the high quality results expected from a sandwich immunoassay. Fully self-contained kits are supplied in convenient 96-well packs. Simple to use and highly sensitive PhosphoTracer kits are designed to get results, fast.
Abcam's PhosphoTracer JNKassay kits detect endogenous levels of JNK 1/2/3 (GenBank Accessions NP_620637 [JNK1], NP_620707 [JNK2] and NP_620446 [JNK3]) in cellular lysates. The phospho-JNK assays detects JNK only when phosphorylated at Thr183/Tyr185. The total JNK assay detects JNK irrespective of phosphorylation.
The substrate used with the HRP conjugated detection antibody is a combination of 10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) (wavelength exc/em = 530-540nm / 590-600nm), a highly sensitive and stable substrate for HRP) and ADHP Dilution Buffer (a stabilized H2O2 solution). Learn more about the fluorogenic substrate, ADHP.
Sensitivity: Phospho-JNK: 50ng/ml (tested with rhJNK1alpha), JNK: 5ng/ml (tested with rhJNK1alpha).
Range: Phospho-JNK: 50-5000ng/ml (tested with rhJNK1alpha), JNK: 5-5000ng/ml (tested with rhJNK1alpha).
|Components||1 x 96 tests|
|96-well PhosphoTracer assay plate (stripwell)||1 unit|
|Adherent plate seal||2 units|
|ADHP (100X)||1 x 120µl|
|ADHP Dilution Buffer||1 x 15ml|
|Assay Control Lysate (lyophilized)||1 x 0.25ml|
|Enhancer Solution||1 x 1ml|
|Lysis Buffer (5X)||1 x 15ml|
|Mouse monoclonal JNK 1/2/3 (HRP) (24 assay points)||1 x 0.75ml|
|Mouse monoclonal Phospho-JNK 1/2/3 (HRP) (72 assay points)||3 x 0.75ml|
|Rabbit monoclonal Phospho-JNK (Thr183/Tyr185)(81E11) (72 assay points)||3 x 0.75ml|
|Rabbit polyclonal JNK 1/2/3 (24 assay points)||1 x 0.75ml|
|Stop Solution||1 x 2ml|
|Wash Buffer (10X)||1 x 15ml|
Our Abpromise guarantee covers the use of ab119663 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration. Phospho-JNK: Tested in HeLa, HEK293, U937, NIH3T3. JNK: Tested in HEK293, HeLa, A431, C2C12, Raw264.7, A549, PC3, NIH3T3, U937.|
ab119663 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"