Overview

  • Product nameAnti-JNK1 antibody [EPR140(2)]
    See all JNK1 primary antibodies
  • Description
    Rabbit monoclonal [EPR140(2)] to JNK1
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human JNK1.

  • Positive control
    • HeLa, 293T, K562, and MCF7 cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab110724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
  • Application notesIs unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • FunctionResponds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
      JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
    • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
      Contains 1 protein kinase domain.
    • DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
    • Post-translational
      modifications
      Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.
    • Information by UniProt
    • Database links
    • Alternative names
      • AI849689 antibody
      • c Jun N terminal kinase 1 antibody
      • C-JUN kinase 1 antibody
      • c-Jun N-terminal kinase 1 antibody
      • EC 2.7.11.24 antibody
      • JNK 1 antibody
      • JNK antibody
      • JNK-46 antibody
      • JNK1A2 antibody
      • JNK21B1/2 antibody
      • MAP kinase 8 antibody
      • MAPK 8 antibody
      • mapk8 antibody
      • Mitogen activated protein kinase 8 antibody
      • Mitogen-activated protein kinase 8 antibody
      • MK08_HUMAN antibody
      • p54 gamma antibody
      • Prkm8 antibody
      • Protein kinase JNK1 antibody
      • Protein kinase, mitogen-activated, 8 antibody
      • SAPK 1 antibody
      • SAPK gamma antibody
      • SAPK1 antibody
      • Stress-activated protein kinase 1 antibody
      • Stress-activated protein kinase JNK1 antibody
      see all

    Anti-JNK1 antibody [EPR140(2)] images



    • Predicted band size : 48 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: JNK1 knockout HAP1 cell lysate (20 µg)

      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: MCF7 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab110724 observed at 46 and 54 kDa. Red - loading control, ab8226, observed at 42 kDa.
      ab110724 was shown to specifically react with JNK1 when JNK1 knockout samples were used. Wild-type and ProteinX knockout samples were subjected to SDS-PAGE. ab110724 and ab8226 (loading control to beta actin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-JNK1 antibody [EPR140(2)] (ab110724) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 48 kDa

    References for Anti-JNK1 antibody [EPR140(2)] (ab110724)

    This product has been referenced in:
    • Zhai W  et al. A1 adenosine receptor attenuates intracerebral hemorrhage-induced secondary brain injury in rats by activating the P38-MAPKAP2-Hsp27 pathway. Mol Brain 9:66 (2016). WB ; Rat . Read more (PubMed: 27301321) »
    • Ye S  et al. Thrombosis recanalization by paeoniflorin through the upregulation of urokinase-type plasminogen activator via the MAPK signaling pathway. Mol Med Rep 13:4593-8 (2016). WB ; Human . Read more (PubMed: 27082639) »

    See all 9 Publications for this product

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