• Product nameAnti-JNK1 antibody [EPR140(2)]
    See all JNK1 primary antibodies
  • Description
    Rabbit monoclonal [EPR140(2)] to JNK1
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human JNK1.

  • Positive control
    • HeLa, 293T, K562, and MCF7 cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.



Our Abpromise guarantee covers the use of ab110724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
  • Application notesIs unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • FunctionResponds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
      JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
    • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
      Contains 1 protein kinase domain.
    • DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
    • Post-translational
      Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.
    • Information by UniProt
    • Database links
    • Alternative names
      • AI849689 antibody
      • c Jun N terminal kinase 1 antibody
      • C-JUN kinase 1 antibody
      • c-Jun N-terminal kinase 1 antibody
      • EC antibody
      • JNK 1 antibody
      • JNK antibody
      • JNK-46 antibody
      • JNK1A2 antibody
      • JNK21B1/2 antibody
      • MAP kinase 8 antibody
      • MAPK 8 antibody
      • mapk8 antibody
      • Mitogen activated protein kinase 8 antibody
      • Mitogen-activated protein kinase 8 antibody
      • MK08_HUMAN antibody
      • p54 gamma antibody
      • Prkm8 antibody
      • Protein kinase JNK1 antibody
      • Protein kinase, mitogen-activated, 8 antibody
      • SAPK 1 antibody
      • SAPK gamma antibody
      • SAPK1 antibody
      • Stress-activated protein kinase 1 antibody
      • Stress-activated protein kinase JNK1 antibody
      see all

    Anti-JNK1 antibody [EPR140(2)] images

    • Predicted band size : 48 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: JNK1 knockout HAP1 cell lysate (20 µg)

      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: MCF7 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab110724 observed at 46 and 54 kDa. Red - loading control, ab8226, observed at 42 kDa.
      ab110724 was shown to specifically react with JNK1 when JNK1 knockout samples were used. Wild-type and ProteinX knockout samples were subjected to SDS-PAGE. ab110724 and ab8226 (loading control to beta actin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-JNK1 antibody [EPR140(2)] (ab110724) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 48 kDa

    References for Anti-JNK1 antibody [EPR140(2)] (ab110724)

    This product has been referenced in:
    • Zhai W  et al. A1 adenosine receptor attenuates intracerebral hemorrhage-induced secondary brain injury in rats by activating the P38-MAPKAP2-Hsp27 pathway. Mol Brain 9:66 (2016). WB ; Rat . Read more (PubMed: 27301321) »
    • Ye S  et al. Thrombosis recanalization by paeoniflorin through the upregulation of urokinase-type plasminogen activator via the MAPK signaling pathway. Mol Med Rep 13:4593-8 (2016). WB ; Human . Read more (PubMed: 27082639) »

    See all 9 Publications for this product

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