Overview

  • Product name
    Anti-JNK1 (phospho T183) antibody
    See all JNK1 primary antibodies
  • Description
    Rabbit polyclonal to JNK1 (phospho T183)
  • Specificity
    The region of JNK1 surrounding T183 has a high degree of similarity to the corresponding regions in JNK2 and JNK3 and thus may cross react with these proteins if phosphorylated on the corresponding residues.
  • Tested applications
    Suitable for: WB, ICC/IF, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthesized phosphopeptide derived from human JNK1 around the phosphorylation site of threonine 183 (M-M-TP-P-Y)

  • Positive control
    • Extracts from Hela cells and human breast carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab47337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 23226105
ELISA 1/4000.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
    JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Domain
    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications
    Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI849689 antibody
    • c Jun N terminal kinase 1 antibody
    • C-JUN kinase 1 antibody
    • c-Jun N-terminal kinase 1 antibody
    • EC 2.7.11.24 antibody
    • JNK 1 antibody
    • JNK antibody
    • JNK-46 antibody
    • JNK1A2 antibody
    • JNK21B1/2 antibody
    • MAP kinase 8 antibody
    • MAPK 8 antibody
    • mapk8 antibody
    • Mitogen activated protein kinase 8 antibody
    • Mitogen-activated protein kinase 8 antibody
    • MK08_HUMAN antibody
    • p54 gamma antibody
    • Prkm8 antibody
    • Protein kinase JNK1 antibody
    • Protein kinase, mitogen-activated, 8 antibody
    • SAPK 1 antibody
    • SAPK gamma antibody
    • SAPK1 antibody
    • Stress-activated protein kinase 1 antibody
    • Stress-activated protein kinase JNK1 antibody
    see all

Anti-JNK1 (phospho T183) antibody images

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab47337 at a 1/50 dilution.

    Left image : Un-treated

    Right image : Treated with P-peptide

  • Immunocytochemistry/ Immunofluorescence analysis of N1E-115 neuroblastoma cells (rescued with different exogenously expressed MKK7-GFP constructs) labeling JNK1 (phospho T183) with ab47337. Quantification of pJNK T183 signals in the neurite.

    N1E-115 cells were washed with PBS, fixed in 80 mM PIPES, 1 mM MgCl2, 1 mM EGTA, pH 6.8 containing 0.25% glutaraldehyde for 45 s and permeabilized in the same buffer containing 0.1% Triton-X for 10 min. Coverslips were incubated with 0.2% sodium borohydride in PBS for 20 min, and blocked in 2% BSA, 0.1% Triton-X in PBS for 15 min. Cells were stained with Anti-JNK1 (phospho T183) antibody (ab47337) for 1 h, and then with secondary antibodies for 30 min (Alexa-fluor 488 labeled phalloidin, Alexa-fluor 546 secondary antibody, and DAPI for 30 min).

  • Immunocytochemistry/ Immunofluorescence analysis of N1E-115 neuroblastoma cells labeling JNK1 (phospho T183) with ab47337. Arrowhead points to high pJNK T183 signal in the neurite shaft, ab47337 specifically stained the neurite.

    N1E-115 cells were washed with PBS, fixed in 80 mM PIPES, 1 mM MgCl2, 1 mM EGTA, pH 6.8 containing 0.25% glutaraldehyde for 45 s and permeabilized in the same buffer containing 0.1% Triton-X for 10 min. Coverslips were incubated with 0.2% sodium borohydride in PBS for 20 min, and blocked in 2% BSA, 0.1% Triton-X in PBS for 15 min. Cells were stained with Anti-JNK1 (phospho T183) antibody (ab47337) for 1 h, and then with secondary antibodies for 30 min (Alexa-fluor 488 labeled phalloidin, Alexa-fluor 546 secondary antibody, and DAPI for 30 min).

  • All lanes : Anti-JNK1 (phospho T183) antibody (ab47337)

    Lane 1 : Extract of Hela cells treated with Anisomycin (200ng/ml, 10min)
    Lane 2 : Extract of Hela cells, untreated


    Predicted band size : 48 kDa

References for Anti-JNK1 (phospho T183) antibody (ab47337)

This product has been referenced in:
  • Ye M  et al. Curcumin Improves Palmitate-Induced Insulin Resistance in Human Umbilical Vein Endothelial Cells by Maintaining Proteostasis in Endoplasmic Reticulum. Front Pharmacol 8:148 (2017). Read more (PubMed: 28377722) »
  • Zhai W  et al. A1 adenosine receptor attenuates intracerebral hemorrhage-induced secondary brain injury in rats by activating the P38-MAPKAP2-Hsp27 pathway. Mol Brain 9:66 (2016). Rat . Read more (PubMed: 27301321) »

See all 9 Publications for this product

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