Anti-JNK1 + JNK2 antibody (ab112501)
Key features and details
- Rabbit polyclonal to JNK1 + JNK2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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Overview
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Product name
Anti-JNK1 + JNK2 antibody
See all JNK1 + JNK2 primary antibodies -
Description
Rabbit polyclonal to JNK1 + JNK2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human JNK1 + JNK2 (internal sequence).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab112501 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 48 kDa.
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IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 48 kDa. |
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
Domain
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
Post-translational
modificationsDually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme. - Information by UniProt
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Database links
- Entrez Gene: 5599 Human
- Entrez Gene: 5601 Human
- Entrez Gene: 26419 Mouse
- Entrez Gene: 26420 Mouse
- Entrez Gene: 116554 Rat
- Entrez Gene: 50658 Rat
- Omim: 601158 Human
- Omim: 602896 Human
see all -
Alternative names
- c jun N terminal kinase 2 antibody
- c-Jun N-terminal kinase 1 antibody
- cJun N terminal kinase 1 antibody
see all
Images
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All lanes : Anti-JNK1 + JNK2 antibody (ab112501)
Lane 1 : Rat brain tissue lysate
Lane 2 : Rat thymus tissue lysate
Lane 3 : MCF-7 whole cell lysate
Lane 4 : HeLa whole cell lysate
Lane 5 : Jurkat whole cell lysate
Lane 6 : MM231 whole cell lysate
Lane 7 : 6T-CEM whole cell lysate
Predicted band size: 48 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted? -
ab112501 staining JNK1+JNK2 in Human intestinal cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
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ab112501 staining JNK1+JNK2 in Rat intestinal tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (31)
ab112501 has been referenced in 31 publications.
- Chang HX & Zhao YF Protocatechuic acid as an inhibitor of the JNK/CXCL1/CXCR2 pathway relieves neuropathic pain in CCI rats. Bosn J Basic Med Sci 22:217-228 (2022). PubMed: 34813418
- Zhou Q et al. Inhibition of AEBP1 predisposes cisplatin-resistant oral cancer cells to ferroptosis. BMC Oral Health 22:478 (2022). PubMed: 36352396
- Wang Y et al. HPV16 E6E7 up-regulates KIF2A expression by activating JNK/c-Jun signal, is beneficial to migration and invasion of cervical cancer cells. Open Med (Wars) 17:1780-1787 (2022). PubMed: 36447525
- Chang C et al. Regulatory role of the TLR4/JNK signaling pathway in sepsis-induced myocardial dysfunction. Mol Med Rep 23:N/A (2021). PubMed: 33760172
- Qu Y et al. The neuroprotection of deproteinized calf blood extractives injection against Alzheimer's disease via regulation of Nrf-2 signaling. Aging (Albany NY) 13:11150-11169 (2021). PubMed: 33819182