• Product nameAnti-JNK1+JNK2 antibody
    See all JNK1+JNK2 primary antibodies
  • Description
    Rabbit polyclonal to JNK1+JNK2
  • Tested applicationsSuitable for: WB, IHC-P, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to an internal sequence of Human JNK1+JNK2.



Our Abpromise guarantee covers the use of ab112501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC Use a concentration of 1 µg/ml.


  • FunctionResponds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
    JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.
  • Information by UniProt
  • Database links
  • Alternative names
    • c jun N terminal kinase 2 antibody
    • c-Jun N-terminal kinase 1 antibody
    • cJun N terminal kinase 1 antibody
    • JNK 1 antibody
    • JNK 2 antibody
    • JNK-46 antibody
    • JNK2ALPHA antibody
    • JNK2BETA antibody
    • MAP kinase 8 antibody
    • MAP kinase 9 antibody
    • MAPK 8 antibody
    • mapk8 antibody
    • MAPK9 antibody
    • Mitogen activated protein kinase 8 antibody
    • Mitogen activated protein kinase 9 antibody
    • Mitogen-activated protein kinase 8 antibody
    • MK08_HUMAN antibody
    • PRKM 8 antibody
    • PRKM 9 antibody
    • Stress-activated protein kinase 1 antibody
    • Stress-activated protein kinase JNK1 antibody
    see all

Anti-JNK1+JNK2 antibody images

  • All lanes : Anti-JNK1+JNK2 antibody (ab112501)

    Lane 1 : Rat brain tissue lysate
    Lane 2 : Rat thymus tissue lysate
    Lane 3 : MCF-7 whole cell lysate
    Lane 4 : HeLa whole cell lysate
    Lane 5 : Jurkat whole cell lysate
    Lane 6 : MM321 whole cell lysate
    Lane 7 : 6T-CEM whole cell lysate

    Predicted band size : 48 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)

References for Anti-JNK1+JNK2 antibody (ab112501)

This product has been referenced in:
  • Ye M  et al. Curcumin Improves Palmitate-Induced Insulin Resistance in Human Umbilical Vein Endothelial Cells by Maintaining Proteostasis in Endoplasmic Reticulum. Front Pharmacol 8:148 (2017). Read more (PubMed: 28377722) »
  • McClelland Descalzo DL  et al. Glucose-Induced Oxidative Stress Reduces Proliferation in Embryonic Stem Cells via FOXO3A/ß-Catenin-Dependent Transcription of p21(cip1). Stem Cell Reports 7:55-68 (2016). WB ; Mouse . Read more (PubMed: 27411103) »

See all 3 Publications for this product

Product Wall

When making quantitative comparisons, such as standardizing the levels of phospho-JNK to total JNK, we do not recommend stripping and reprobing the membrane, as stripping removes some sample protein from the membrane. Instead, you should either ru...

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