Overview

  • Product nameAnti-JNK1+JNK2 antibody
    See all JNK1+JNK2 primary antibodies
  • Description
    Rabbit polyclonal to JNK1+JNK2
  • Tested applicationsSuitable for: WB, IHC-P, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to an internal sequence of Human JNK1+JNK2.

Properties

Applications

Our Abpromise guarantee covers the use of ab112501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC Use a concentration of 1 µg/ml.

Target

  • FunctionResponds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
    JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications
    Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.
  • Information by UniProt
  • Database links
  • Alternative names
    • c jun N terminal kinase 2 antibody
    • c-Jun N-terminal kinase 1 antibody
    • cJun N terminal kinase 1 antibody
    • JNK 1 antibody
    • JNK 2 antibody
    • JNK-46 antibody
    • JNK2ALPHA antibody
    • JNK2BETA antibody
    • MAP kinase 8 antibody
    • MAP kinase 9 antibody
    • MAPK 8 antibody
    • mapk8 antibody
    • MAPK9 antibody
    • Mitogen activated protein kinase 8 antibody
    • Mitogen activated protein kinase 9 antibody
    • Mitogen-activated protein kinase 8 antibody
    • MK08_HUMAN antibody
    • PRKM 8 antibody
    • PRKM 9 antibody
    • Stress-activated protein kinase 1 antibody
    • Stress-activated protein kinase JNK1 antibody
    see all

Anti-JNK1+JNK2 antibody images

  • All lanes : Anti-JNK1+JNK2 antibody (ab112501)

    Lane 1 : Rat brain tissue lysate
    Lane 2 : Rat thymus tissue lysate
    Lane 3 : MCF-7 whole cell lysate
    Lane 4 : HeLa whole cell lysate
    Lane 5 : Jurkat whole cell lysate
    Lane 6 : MM321 whole cell lysate
    Lane 7 : 6T-CEM whole cell lysate


    Predicted band size : 48 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)

References for Anti-JNK1+JNK2 antibody (ab112501)

This product has been referenced in:
  • Eaton GJ  et al. Inhibition of apoptosis signal-regulating kinase 1 enhances endochondral bone formation by increasing chondrocyte survival. Cell Death Dis 5:e1522 (2014). WB ; Mouse . Read more (PubMed: 25393478) »

See 1 Publication for this product

Product Wall


When making quantitative comparisons, such as standardizing the levels of phospho-JNK to total JNK, we do not recommend stripping and reprobing the membrane, as stripping removes some sample protein from the membrane. Instead, you should either ru...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"