• Product nameAnti-JNK2 antibody
    See all JNK2 primary antibodies
  • Description
    Mouse monoclonal to JNK2
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 321-424 of Human JNK2, with a 26kDa tag. AAH32539.

  • Positive control
    • Recombinant protein, HeLa cell lysate and HeLa cells.


Associated products


Our Abpromise guarantee covers the use of ab88076 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 48 kDa.
ELISA Use at an assay dependent dilution.
ICC/IF Use a concentration of 10 µg/ml.


  • FunctionResponds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells.
    JNK2 isoforms display different binding patterns: alpha-1 and alpha-2 preferentially bind to c-Jun, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme. Autophosphorylated in vitro.
  • Information by UniProt
  • Database links
  • Alternative names
    • c Jun kinase 2 antibody
    • C Jun N terminal kinase 2 antibody
    • c-Jun N-terminal kinase 2 antibody
    • JNK 55 antibody
    • JNK-55 antibody
    • JNK2 alpha antibody
    • JNK2 antibody
    • JNK2 beta antibody
    • JNK2A antibody
    • JNK2alpha antibody
    • JNK2B antibody
    • JNK2BETA antibody
    • Jun kinase antibody
    • MAP kinase 9 antibody
    • MAPK 9 antibody
    • Mapk9 antibody
    • Mitogen activated protein kinase 9 antibody
    • Mitogen-activated protein kinase 9 antibody
    • MK09_HUMAN antibody
    • P54a antibody
    • p54aSAPK antibody
    • PRKM9 antibody
    • SAPK alpha antibody
    • SAPK antibody
    • SAPK1a antibody
    • Stress activated protein kinase 1a antibody
    • Stress-activated protein kinase JNK2 antibody
    see all

Anti-JNK2 antibody images

  • Anti-JNK2 antibody (ab88076) at 5 µg/ml + recombinant protein (immunogen) at 0.2 µg

    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 48 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-JNK2 antibody (ab88076) at 5 µg/ml + Jurkat cell lysate at 25 µg

    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 48 kDa
    Observed band size : 48 kDa
    Additional bands at : 250 kDa,37 kDa. We are unsure as to the identity of these extra bands.
  • ab88076 at 10µg/ml staining JNK2 in Hela cells.
  • Detection limit for ab88076 is approximately 0.03ng/ml as a capture antibody.
  • Proximity Ligation Analysis (PLA) of protein-protein interactions between TRAF2 and JNK2 HeLa cells were stained with anti-TRAF2 rabbit purified polyclonal 1/1200 and ab88076 1/50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.

References for Anti-JNK2 antibody (ab88076)

ab88076 has not yet been referenced specifically in any publications.

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