Overview

  • Product nameAnti-JNK3 antibody
    See all JNK3 primary antibodies
  • Description
    Rabbit polyclonal to JNK3
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human JNK3 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • Positive control
    • Mouse spleen tissue lysate and human breast carcinoma tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab70882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 45 kDa (predicted molecular weight: 53 kDa).

Target

  • FunctionResponds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. Required for stress-induced neuronal apoptosis and the pathogenesis of glutamate excitotoxicity.
  • Tissue specificitySpecific to a subset of neurons in the nervous system. Present in the hippocampus and areas, cerebellum, striatum, brain stem, and weakly in the spinal cord. Very weak expression in testis and kidney.
  • Involvement in diseaseDefects in MAPK10 are a cause of epileptic encephalopathy Lennox-Gastaut type (EELG) [MIM:606369]. Epileptic encephalopathies of the Lennox-Gastaut group are childhood epileptic disorders characterized by severe psychomotor delay and seizures. Note=A chromosomal aberration involving MAPK10 has been found in a single patient. Translocation t(Y;4)(q11.2;q21) which causes MAPK10 truncation.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications
    Dually phosphorylated on Thr-221 and Tyr-223, which activates the enzyme. Weakly autophosphorylated on threonine and tyrosine residues in vitro.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • c Jun kinase 3 antibody
    • c-Jun N-terminal kinase 3 antibody
    • cJun N terminal kinase 3 antibody
    • FLJ12099 antibody
    • FLJ33785 antibody
    • JNK3 alpha protein kinase antibody
    • JNK3 antibody
    • JNK3A antibody
    • MAP kinase 10 antibody
    • MAP kinase antibody
    • MAP kinase p49 3F12 antibody
    • MAPK 10 antibody
    • Mapk10 antibody
    • MGC50974 antibody
    • mitogen activated protein kinase 10 antibody
    • Mitogen-activated protein kinase 10 antibody
    • MK10_HUMAN antibody
    • p493F12 antibody
    • p54bSAPK antibody
    • PRKM10 antibody
    • protein kinase mitogen activated 10 antibody
    • SAPK1b antibody
    • Stress activated protein kinase 1b antibody
    • stress activated protein kinase beta antibody
    • Stress activated protein kinase JNK3 antibody
    • Stress-activated protein kinase JNK3 antibody
    see all

Anti-JNK3 antibody images

  • Anti-JNK3 antibody (ab70882) at 1/100 dilution + mouse spleen tissue lysate at 12.5 µg

    Secondary
    HRP-anti-rabbit

    Predicted band size : 53 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 16 kDa,22 kDa. We are unsure as to the identity of these extra bands.
  • ab70882 at 1/50 dilution, staining JNK3 in human breast carcinoma by Immunohistochemistry, using formalin fixed, paraffin embedded tissue. ab70882 was peroxidase conjugated to the secondary antibody, followed by DAB staining.

References for Anti-JNK3 antibody (ab70882)

ab70882 has not yet been referenced specifically in any publications.

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