Overview

  • Product name
  • Description
    Rabbit polyclonal to JNK3
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human JNK3 aa 300-400 conjugated to Keyhole Limpet Haemocyanin (KLH).
    (Peptide available as ab101295, ab101295)

  • Positive control
    • This antibody gave a positive signal in U87mg whole cell lysate as well as the following tissue lysates: Human brain; Human spinal cord; Mouse hippocampus; Mouse cerebellum; Rat hippocampus; Rat cerebellum.

Properties

Applications

Our Abpromise guarantee covers the use of ab87404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 52 kDa).

Target

  • Function
    Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. Required for stress-induced neuronal apoptosis and the pathogenesis of glutamate excitotoxicity.
  • Tissue specificity
    Specific to a subset of neurons in the nervous system. Present in the hippocampus and areas, cerebellum, striatum, brain stem, and weakly in the spinal cord. Very weak expression in testis and kidney.
  • Involvement in disease
    Defects in MAPK10 are a cause of epileptic encephalopathy Lennox-Gastaut type (EELG) [MIM:606369]. Epileptic encephalopathies of the Lennox-Gastaut group are childhood epileptic disorders characterized by severe psychomotor delay and seizures. Note=A chromosomal aberration involving MAPK10 has been found in a single patient. Translocation t(Y;4)(q11.2;q21) which causes MAPK10 truncation.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Domain
    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications
    Dually phosphorylated on Thr-221 and Tyr-223, which activates the enzyme. Weakly autophosphorylated on threonine and tyrosine residues in vitro.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • c Jun kinase 3 antibody
    • c-Jun N-terminal kinase 3 antibody
    • cJun N terminal kinase 3 antibody
    • FLJ12099 antibody
    • FLJ33785 antibody
    • JNK3 alpha protein kinase antibody
    • JNK3 antibody
    • JNK3A antibody
    • MAP kinase 10 antibody
    • MAP kinase antibody
    • MAP kinase p49 3F12 antibody
    • MAPK 10 antibody
    • Mapk10 antibody
    • MGC50974 antibody
    • mitogen activated protein kinase 10 antibody
    • Mitogen-activated protein kinase 10 antibody
    • MK10_HUMAN antibody
    • p493F12 antibody
    • p54bSAPK antibody
    • PRKM10 antibody
    • protein kinase mitogen activated 10 antibody
    • SAPK1b antibody
    • Stress activated protein kinase 1b antibody
    • stress activated protein kinase beta antibody
    • Stress activated protein kinase JNK3 antibody
    • Stress-activated protein kinase JNK3 antibody
    see all

Images

  • All lanes : Anti-JNK3 antibody (ab87404) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human spinal cord tissue lysate - total protein (ab29188)
    Lane 3 : Mouse Hippocampus Tissue Lysate
    Lane 4 : Cerebellum Mouse Tissue Lysate
    Lane 5 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
    Lane 6 : Rat Hippocampus Tissue Lysate
    Lane 7 : Cerebellum Rat Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size : 52 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 34 kDa,52 kDa. We are unsure as to the identity of these extra bands.The predicted molecular weight of MAPK10 is 52 kDa (SwissProt), however we expect to observe a banding pattern at 48 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

References

ab87404 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Loading amount
5 µg
Gel Running Conditions
Reduced Denaturing
Sample
Anopheles stephensi Cell lysate - whole cell (midgut)
Specification
midgut
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Verified customer

Submitted Apr 11 2014

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