Anti-JPH3 antibody (ab79063)
Key features and details
- Rabbit polyclonal to JPH3
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-JPH3 antibody -
Description
Rabbit polyclonal to JPH3 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
A synthetic 18 amino acid peptide near the carboxy terminus of human JPH3 (protein accession number: NP_065706)
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General notes
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Affinity chromatography purified via peptide column -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab79063 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 20 µg/ml.
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 81 kDa.
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Notes |
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ICC/IF
Use a concentration of 20 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 81 kDa. |
Target
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Function
Contributes to the stabilization of the junctional membrane complexes, which are common to excitable cells and mediate cross-talk between cell surface and intracellular ion channels. Probably acts by anchoring the plasma membrane and endoplasmic reticulum. May play an active role in certain neurons involved in motor coordination. -
Tissue specificity
Specifically expressed in brain. -
Involvement in disease
Defects in JPH3 are the cause of Huntington disease-like type 2 (HDL2) [MIM:606438]. Huntington disease (HD) is a neurodegenerative disorder resulting primarily from the loss of medium spiny projection neurons in the striatum, especially in the caudate nucleus, and, to a lesser extent, atrophy of mesencephalic and cortical structures. The typical clinical picture of HD combines familial adult onset chorea and subcortical dementia that usually begin during the fourth decade of life. -
Sequence similarities
Belongs to the junctophilin family.
Contains 8 MORN repeats. -
Domain
The MORN (membrane occupation and recognition nexus) repeats contribute to the plasma membrane binding, possibly by interacting with phospholipids. -
Cellular localization
Cell membrane. Endoplasmic reticulum membrane. Localized predominantly on the plasma membrane. The transmembrane domain is anchored in endoplasmic reticulum membrane, while the N-terminal part associates with the plasma membrane. - Information by UniProt
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Database links
- Entrez Gene: 57338 Human
- Omim: 605268 Human
- SwissProt: Q8WXH2 Human
- Unigene: 725123 Human
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Alternative names
- JP-3 antibody
- JP3 antibody
- JPH3 antibody
see all
Images
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All lanes : Anti-JPH3 antibody (ab79063) at 1 µg/ml
Lane 1 : Daudi cell lysate
Lane 2 : Daudi cell lysate with blocking peptide
Lysates/proteins at 15 µg per lane.
Predicted band size: 81 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted? -
Immunofluorescence of JPH3 in Human Brain cells using ab79063 at 20 ug/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab79063 has not yet been referenced specifically in any publications.