Overview

  • Product nameAnti-JPH3 antibody
    See all JPH3 primary antibodies
  • Description
    Rabbit polyclonal to JPH3
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide designed within N terminal amino acids 2-51 (SSGGRFNFD DGGSYCGGWE DGKAHGHGVC TGPKGQGEYT GSWSHGFEVL G) of Human JPH3 (NP_065706)

  • Positive control
    • HepG2 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab81178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 81 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

Target

  • FunctionContributes to the stabilization of the junctional membrane complexes, which are common to excitable cells and mediate cross-talk between cell surface and intracellular ion channels. Probably acts by anchoring the plasma membrane and endoplasmic reticulum. May play an active role in certain neurons involved in motor coordination.
  • Tissue specificitySpecifically expressed in brain.
  • Involvement in diseaseDefects in JPH3 are the cause of Huntington disease-like type 2 (HDL2) [MIM:606438]. Huntington disease (HD) is a neurodegenerative disorder resulting primarily from the loss of medium spiny projection neurons in the striatum, especially in the caudate nucleus, and, to a lesser extent, atrophy of mesencephalic and cortical structures. The typical clinical picture of HD combines familial adult onset chorea and subcortical dementia that usually begin during the fourth decade of life.
  • Sequence similaritiesBelongs to the junctophilin family.
    Contains 8 MORN repeats.
  • DomainThe MORN (membrane occupation and recognition nexus) repeats contribute to the plasma membrane binding, possibly by interacting with phospholipids.
  • Cellular localizationCell membrane. Endoplasmic reticulum membrane. Localized predominantly on the plasma membrane. The transmembrane domain is anchored in endoplasmic reticulum membrane, while the N-terminal part associates with the plasma membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • JP-3 antibody
    • JP3 antibody
    • JPH3 antibody
    • JPH3_HUMAN antibody
    • Junctophilin 3 antibody
    • Junctophilin type 3 antibody
    • Junctophilin-3 antibody
    • TNRC22 antibody
    • Trinucleotide repeat-containing gene 22 protein antibody
    see all

Anti-JPH3 antibody images

  • Anti-JPH3 antibody (ab81178) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 81 kDa
    Observed band size : 81 kDa
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.

References for Anti-JPH3 antibody (ab81178)

ab81178 has not yet been referenced specifically in any publications.

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