Overview

  • Product nameAnti-JunB antibody
    See all JunB primary antibodies
  • Description
    Rabbit polyclonal to JunB
  • SpecificityAntibody detects endogenous levels of JunB protein around Serine 259.
  • Tested applicationsSuitable for: ICC/IF, WB, IP, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic non-phosphopeptide derived from Human JunB around the phosphorylation site of Serine 259.

  • Positive control
    • Extracts of Hela cells and breast carcinoma tissue. Hek293 clls.

Properties

Applications

Our Abpromise guarantee covers the use of ab31421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 35 kDa.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.

Target

Anti-JunB antibody images

  • ICC/IF image of ab31421 stained Hek293 cells (ab7902). The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum (ab7481) / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31421, 5µg/ml) overnight at +4°C. The secondary antibody (green) was for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-JunB antibody (ab31421) at 1/500 dilution

    Lane 1 : Extracts of Hela cells.
    Lane 2 : Extracts of Hela cells. Antibody pre-incubated with synthesized peptide.


    Predicted band size : 35 kDa
    Observed band size : 41 kDa (why is the actual band size different from the predicted?)
  • ab31421 at a 1:50-1:100 dilution, staining JunB in human breast carcinoma using Immunohistochemistry, Paraffin Embedded Tissue.

    Left image : Un-treated.

    Right image : Antibody pre-incubated with synthesized peptide.

References for Anti-JunB antibody (ab31421)

This product has been referenced in:
  • Marban C  et al. Interplay between the HTLV-2 Tax and APH-2 proteins in the regulation of the AP-1 pathway. Retrovirology 9:98 (2012). IP ; Human . Read more (PubMed: 23206352) »
  • Raffaello A  et al. JunB transcription factor maintains skeletal muscle mass and promotes hypertrophy. J Cell Biol 191:101-13 (2010). WB ; Mouse . Read more (PubMed: 20921137) »

See all 4 Publications for this product

Product Wall

Application Immunoprecipitation
Immuno-precipitation step Other - EMSA
Sample Human Cell lysate - nuclear (renal cancer cells)
Specification renal cancer cells
Total protein in input 3 µg
Username

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Verified customer

Submitted Dec 27 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"