JunB overexpression 293T lysate (whole cell) (ab94280)
Overview
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Product name
JunB overexpression 293T lysate (whole cell)
See all JunB lysates -
General notes
ab94280 is a 293T cell transfected lysate in which Human JunB has been transiently over-expressed using a pCMV-JunB plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Research areas
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Background
Function: Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'. Similarity: Belongs to the bZIP family. Jun subfamily. Contains 1 bZIP domain. PTM: Ubiquitinated by ITCH, leading to its degradation.
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab94280 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent dilution.
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Notes |
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WB
Use at an assay dependent dilution. |
Images
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ab94280 at 15µg/lane on an SDS-PAGE gel.
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All lanes : Anti-JunB antibody (ab90565) at 1/500 dilution
Lane 1 :JunB overexpression 293T lysate (whole cell) (ab94280)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab94280 has not yet been referenced specifically in any publications.