JunB overexpression 293T lysate (whole cell) (ab94280)

Overview

  • Product nameJunB overexpression 293T lysate (whole cell)
  • Description
    JunB overexpression lysate
  • General notesab94280 is a 293T cell transfected lysate in which Human JunB has been transiently over-expressed using a pCMV-JunB plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundFunction: Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'. Similarity: Belongs to the bZIP family. Jun subfamily. Contains 1 bZIP domain. PTM: Ubiquitinated by ITCH, leading to its degradation.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

JunB overexpression 293T lysate (whole cell) images

  • ab94280 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-JunB antibody (ab90565) at 1/500 dilution

    Lane 1 : JunB overexpression 293T lysate (whole cell) (ab94280)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for JunB overexpression 293T lysate (whole cell) (ab94280)

ab94280 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"