Overview

  • Product nameAnti-JunB (phospho S259) antibody
    See all JunB primary antibodies
  • Description
    Rabbit polyclonal to JunB (phospho S259)
  • Specificityab30628 recognises endogenous levels of JunB only when phosphorylated at Serine 259.
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from human JunB around the phosphorylation site of Serine 259.

  • Positive control
    • Breast carcinoma, HeLa cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab30628 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 35 kDa.
ELISA 1/20000.
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

Anti-JunB (phospho S259) antibody images

  • All lanes : Anti-JunB (phospho S259) antibody (ab30628) at 1/500 dilution

    Lane 1 : extracts from HeLa cells (5-30ug).
    Lane 2 : extracts from HeLa cells (5-30ug), preincubated with synthesized non phosphopeptide.
    Lane 3 : extracts from HeLa cells (5-30ug), preincubated with synthesized phosphopeptide (negative control).


    Predicted band size : 35 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of paraffin embedded breast carcinoma tissue sections, using 1/50 JunB (Phospho Ser259) Antibody (ab30628). Left: untreated sample; Right: sample preincubated with synthesized phosphopeptide (negative control).

References for Anti-JunB (phospho S259) antibody (ab30628)

This product has been referenced in:
  • Suwei D  et al. NLK functions to maintain proliferation and stemness of NSCLC and is a target of metformin. J Hematol Oncol 8:120 (2015). Human . Read more (PubMed: 26503334) »
  • Pérez-Benavente B  et al. GSK3-SCF(FBXW7) targets JunB for degradation in G2 to preserve chromatid cohesion before anaphase. Oncogene : (2012). Read more (PubMed: 22710716) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"