Anti-Junctional Adhesion Molecule 1 antibody (ab125886)

Overview

  • Product nameAnti-Junctional Adhesion Molecule 1 antibody
    See all Junctional Adhesion Molecule 1 primary antibodies
  • Description
    Rabbit polyclonal to Junctional Adhesion Molecule 1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Chinese Hamster
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Mouse Junctional Adhesion Molecule 1.

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Mouse Brain; Rat Brain; Mouse Cortex; Rat Cortex; Mouse Cerebellum; Mouse Spinal Cord.

Properties

Applications

Our Abpromise guarantee covers the use of ab125886 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 32 kDa).

Target

  • FunctionSeems to plays a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus.
  • Sequence similaritiesBelongs to the immunoglobulin superfamily.
    Contains 2 Ig-like V-type (immunoglobulin-like) domains.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localizationCell junction > tight junction. Cell membrane. Localized at tight junctions of both epithelial and endothelial cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 321 antibody
    • CD321 antibody
    • CD321 antigen antibody
    • ESTM33 antibody
    • F11 receptor antibody
    • F11R antibody
    • JAM 1 antibody
    • JAM A antibody
    • JAM antibody
    • JAM-1 antibody
    • JAM-A antibody
    • JAM1 antibody
    • JAM1_HUMAN antibody
    • JAMA antibody
    • JCAM antibody
    • Jcam1 antibody
    • Junction adhesion molecule 1 antibody
    • Junction adhesion molecule, mouse, homolog of antibody
    • Junctional adhesion molecule 1 antibody
    • Junctional adhesion molecule A antibody
    • KAT antibody
    • Ly106 antibody
    • PAM 1 antibody
    • PAM-1 antibody
    • PAM1 antibody
    • Platelet adhesion molecule 1 antibody
    • Platelet adhesion molecule antibody
    • Platelet F11 receptor antibody
    • PRO301 antibody
    • UNQ264 antibody
    see all

Anti-Junctional Adhesion Molecule 1 antibody images

  • All lanes : Anti-Junctional Adhesion Molecule 1 antibody (ab125886) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Brain (Rat) Tissue Lysate
    Lane 3 : Mouse Cortex Tissue Lysate
    Lane 4 : Rat Cortex Tissue Lysate
    Lane 5 : Cerebellum Mouse Tissue Lysate
    Lane 6 : Spinal Cord (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 32 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 53 kDa,99 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

References for Anti-Junctional Adhesion Molecule 1 antibody (ab125886)

ab125886 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (intestinal tissue)
Gel Running Conditions Reduced Denaturing (4-12% Bis Tris MOPS)
Loading amount 10 µg
Specification intestinal tissue
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Oct 20 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"