Recombinant
RabMAb

Anti-Junctional Adhesion Molecule 1 antibody [EP1042Y] (ab52647)

Overview

  • Product name
    Anti-Junctional Adhesion Molecule 1 antibody [EP1042Y]
    See all Junctional Adhesion Molecule 1 primary antibodies
  • Description
    Rabbit monoclonal [EP1042Y] to Junctional Adhesion Molecule 1
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues near the N-terminus of human Junctional Adhesion Molecule 1.

  • Positive control
    • Hela cell lysate and human liver tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab52647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
IHC-P 1/100 - 1/250.
  • Application notes
    Is unsuitable for ICC.
  • Target

    • Function
      Seems to plays a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus.
    • Sequence similarities
      Belongs to the immunoglobulin superfamily.
      Contains 2 Ig-like V-type (immunoglobulin-like) domains.
    • Post-translational
      modifications
      N-glycosylated.
    • Cellular localization
      Cell junction > tight junction. Cell membrane. Localized at tight junctions of both epithelial and endothelial cells.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 321 antibody
      • CD321 antibody
      • CD321 antigen antibody
      • ESTM33 antibody
      • F11 receptor antibody
      • F11R antibody
      • JAM 1 antibody
      • JAM A antibody
      • JAM antibody
      • JAM-1 antibody
      • JAM-A antibody
      • JAM1 antibody
      • JAM1_HUMAN antibody
      • JAMA antibody
      • JCAM antibody
      • Jcam1 antibody
      • Junction adhesion molecule 1 antibody
      • Junction adhesion molecule, mouse, homolog of antibody
      • Junctional adhesion molecule 1 antibody
      • Junctional adhesion molecule A antibody
      • KAT antibody
      • Ly106 antibody
      • PAM 1 antibody
      • PAM-1 antibody
      • PAM1 antibody
      • Platelet adhesion molecule 1 antibody
      • Platelet adhesion molecule antibody
      • Platelet F11 receptor antibody
      • PRO301 antibody
      • UNQ264 antibody
      see all

    Images

    • Anti-Junctional Adhesion Molecule 1 antibody [EP1042Y] (ab52647) at 1/2000 dilution (unpurified) + Hela cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 33 kDa
      Observed band size : 33 kDa
    • Immunohistochemical staining of paraffin-embedded human liver using unpurified ab52647 at a 1/100 dilution.

    References

    This product has been referenced in:
    • Sborov DW  et al. A phase I trial of single-agent reolysin in patients with relapsed multiple myeloma. Clin Cancer Res 20:5946-55 (2014). Read more (PubMed: 25294913) »
    • Manetti M  et al. Differential expression of junctional adhesion molecules in different stages of systemic sclerosis. Arthritis Rheum 65:247-57 (2013). IHC . Read more (PubMed: 23001478) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Intestinal tissue)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis Tris MOPS)
    Loading amount
    14 µg
    Specification
    Intestinal tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Mr. Julien Chevalier

    Verified customer

    Submitted Nov 18 2015

    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Intestinal tissue (mucosa and submucosa))
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis Tris MOPS)
    Loading amount
    14 µg
    Specification
    Intestinal tissue (mucosa and submucosa)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Mr. Julien Chevalier

    Verified customer

    Submitted Oct 16 2015

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Intestinal tissue)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis Tris MOPS)
    Loading amount
    14 µg
    Specification
    Intestinal tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Mr. Julien Chevalier

    Verified customer

    Submitted Oct 12 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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