Anti-Junctional Adhesion Molecule 1 antibody [EP1042Y] (ab52647)

Overview

  • Product nameAnti-Junctional Adhesion Molecule 1 antibody [EP1042Y]
    See all Junctional Adhesion Molecule 1 primary antibodies
  • Description
    Rabbit monoclonal [EP1042Y] to Junctional Adhesion Molecule 1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the N-terminus of human Junctional Adhesion Molecule 1.

  • Positive control
    • Hela cell lysate and human liver tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab52647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
IHC-P 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt,ICC or IP.
  • Target

    • FunctionSeems to plays a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus.
    • Sequence similaritiesBelongs to the immunoglobulin superfamily.
      Contains 2 Ig-like V-type (immunoglobulin-like) domains.
    • Post-translational
      modifications
      N-glycosylated.
    • Cellular localizationCell junction > tight junction. Cell membrane. Localized at tight junctions of both epithelial and endothelial cells.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 321 antibody
      • CD321 antibody
      • CD321 antigen antibody
      • ESTM33 antibody
      • F11 receptor antibody
      • F11R antibody
      • JAM 1 antibody
      • JAM A antibody
      • JAM antibody
      • JAM-1 antibody
      • JAM-A antibody
      • JAM1 antibody
      • JAM1_HUMAN antibody
      • JAMA antibody
      • JCAM antibody
      • Jcam1 antibody
      • Junction adhesion molecule 1 antibody
      • Junction adhesion molecule, mouse, homolog of antibody
      • Junctional adhesion molecule 1 antibody
      • Junctional adhesion molecule A antibody
      • KAT antibody
      • Ly106 antibody
      • PAM 1 antibody
      • PAM-1 antibody
      • PAM1 antibody
      • Platelet adhesion molecule 1 antibody
      • Platelet adhesion molecule antibody
      • Platelet F11 receptor antibody
      • PRO301 antibody
      • UNQ264 antibody
      see all

    Anti-Junctional Adhesion Molecule 1 antibody [EP1042Y] images

    • Anti-Junctional Adhesion Molecule 1 antibody [EP1042Y] (ab52647) at 1/2000 dilution + Hela cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 33 kDa
      Observed band size : 33 kDa
    • Immunohistochemical staining of paraffin-embedded human liver using ab52647 at a 1/100 dilution.

    References for Anti-Junctional Adhesion Molecule 1 antibody [EP1042Y] (ab52647)

    This product has been referenced in:
    • Sborov DW  et al. A phase I trial of single-agent reolysin in patients with relapsed multiple myeloma. Clin Cancer Res 20:5946-55 (2014). Read more (PubMed: 25294913) »
    • Manetti M  et al. Differential expression of junctional adhesion molecules in different stages of systemic sclerosis. Arthritis Rheum 65:247-57 (2013). IHC . Read more (PubMed: 23001478) »

    See all 3 Publications for this product

    Product Wall

    Application Western blot
    Sample Rat Tissue lysate - whole (Intestinal tissue)
    Gel Running Conditions Reduced Denaturing (4-12% Bis Tris MOPS)
    Loading amount 14 µg
    Specification Intestinal tissue
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Mr. Julien Chevalier

    Verified customer

    Submitted Nov 18 2015

    Application Western blot
    Sample Human Tissue lysate - whole (Intestinal tissue (mucosa and submucosa))
    Gel Running Conditions Reduced Denaturing (4-12% Bis Tris MOPS)
    Loading amount 14 µg
    Specification Intestinal tissue (mucosa and submucosa)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Mr. Julien Chevalier

    Verified customer

    Submitted Oct 16 2015

    Application Western blot
    Sample Mouse Tissue lysate - whole (Intestinal tissue)
    Gel Running Conditions Reduced Denaturing (4-12% Bis Tris MOPS)
    Loading amount 14 µg
    Specification Intestinal tissue
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Mr. Julien Chevalier

    Verified customer

    Submitted Oct 12 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"