Recombinant
RabMAb

Anti-Junctional Adhesion Molecule 2 antibody [EPR2489(2)] (ab156586)

Overview

  • Product name
    Anti-Junctional Adhesion Molecule 2 antibody [EPR2489(2)]
    See all Junctional Adhesion Molecule 2 primary antibodies
  • Description
    Rabbit monoclonal [EPR2489(2)] to Junctional Adhesion Molecule 2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human Junctional Adhesion Molecule 2 (UniProt ID: P57087).

  • Positive control
    • HUVEC, HeLa, JAR and A431 cell lysates, A431 cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab156586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 33 kDa.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function
      May play a role in the processes of lymphocyte homing to secondary lymphoid organs.
    • Tissue specificity
      Highest expression in the heart, placenta, lung, foreskin and lymph node. Prominently expressed on high endothelial venules, also present on the endothelia of other vessels. Localized to the intercellular boundaries of high endothelial cells.
    • Sequence similarities
      Belongs to the immunoglobulin superfamily.
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Cellular localization
      Cell junction > tight junction. Cell membrane. Localized at tight junctions of both epithelial and endothelial cells.
    • Information by UniProt
    • Database links
    • Alternative names
      • C21orf43 antibody
      • CD 322 antibody
      • CD322 antibody
      • CD322 antigen antibody
      • JAM 2 antibody
      • JAM B antibody
      • JAM IT/VE JAM antibody
      • JAM, vascular endothelial antibody
      • JAM-2 antibody
      • JAM-B antibody
      • JAM2 antibody
      • JAM2_HUMAN antibody
      • JAMB antibody
      • Jcam2 antibody
      • Junction adhesion molecule 2 antibody
      • Junction adhesion molecule B antibody
      • Junctional adhesion molecule 2 antibody
      • Junctional adhesion molecule B antibody
      • PRO 245 antibody
      • PRO245 antibody
      • Vascular endothelial junction associated molecule antibody
      • Vascular endothelial junction-associated molecule antibody
      • VE JAM antibody
      • VE-JAM antibody
      • VEJAM antibody
      see all

    Images

    • All lanes : Anti-Junctional Adhesion Molecule 2 antibody [EPR2489(2)] (ab156586) at 1/1000 dilution

      Lane 1 : HUVEC cell lysates
      Lane 2 : HeLa cell lysates
      Lane 3 : JAR cell lysates
      Lane 4 : A431 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 33 kDa

    • Flow cytometric analysis of permeabilized A431 cells labeling Junctional Adhesion Molecule 2 using ab156586 at a 1/100 dilution (red) or a rabbit IgG control (green).

    References

    ab156586 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunoprecipitation
    Immuno-precipitation step
    Protein A/G
    Sample
    Mouse Cell lysate - other (fibroblast)
    Specification
    fibroblast
    Total protein in input
    100 µg
    Username

    Sir or Madam Dwscott

    Verified customer

    Submitted Jul 26 2013

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Sample
    Mouse Cell (fibroblast)
    Specification
    fibroblast
    Permeabilization
    Yes - 0.1% Triton X-100, 5 min
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jul 23 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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