Anti-JAM-C antibody [19 H36] (ab16889)
Key features and details
- Rat monoclonal [19 H36] to JAM-C
- Suitable for: IHC-Fr, IP, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG2a
Overview
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Product name
Anti-JAM-C antibody [19 H36]
See all JAM-C primary antibodies -
Description
Rat monoclonal [19 H36] to JAM-C -
Host species
Rat -
Tested applications
Suitable for: IHC-Fr, IP, Flow Cytmore details
Unsuitable for: IHC-P or WB -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- High endothelial venules of lymphoid organs, lymphoendothelial cells and endothelial cells of the kidney
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
19 H36 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab16889 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Notes |
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IHC-Fr
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Target
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Function
Participates in cell-cell adhesion. It is a counterreceptor for ITGAM, mediating leukocyte-platelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN). The soluble form is a mediator of angiogenesis. -
Tissue specificity
Highest expression in placenta, brain and kidney. Significant expression is detected on platelets. Expressed in intestinal mucosa cells. Expressed in the vascular endothelium. Found in serum (at protein level). Also detected in the synovial fluid of patients with rheumatoid arthritis, psoriatic arthritis or ostearthritis (at protein level). -
Involvement in disease
Defects in JAM3 are the cause of hemorrhagic destruction of the brain with subependymal calcification and cataracts (HDBSCC) [MIM:613730]. A syndrome characterized by congenital cataracts and severe brain abnormalities apparently resulting from hemorrhagic destruction of the brain tissue, including the cerebral white matter and basal ganglia. Patients manifest profound developmental delay, and other neurologic features included seizures, spasticity, and hyperreflexia. Brain imaging shows multifocal intraparenchymal hemorrhage with associated liquefaction and massive cystic degeneration, and calcification in the subependymal region and in brain tissue. -
Sequence similarities
Belongs to the immunoglobulin superfamily.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsProteolytically cleaved from endothelial cells surface into a soluble form by ADAM10 and ADAM17; the release of soluble JAM3 is increased by proinflammatory factors. -
Cellular localization
Cell membrane. Cell junction > desmosome. Secreted > extracellular space. In epithelial cells, it is expressed at desmosomes but not at tight junctions. Localizes at the cell surface of endothelial cells; treatment of endothelial cells with vascular endothelial growth factor stimulates recruitement of JAM3 to cell-cell contacts. - Information by UniProt
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Database links
- Entrez Gene: 83700 Human
- Entrez Gene: 83964 Mouse
- Omim: 606871 Human
- SwissProt: Q9BX67 Human
- SwissProt: Q9D8B7 Mouse
- Unigene: 150718 Human
- Unigene: 728339 Human
- Unigene: 28770 Mouse
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Alternative names
- FLJ14529 antibody
- JAM 2 antibody
- JAM 3 antibody
see all
Datasheets and documents
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Datasheet download
References (0)
ab16889 has not yet been referenced specifically in any publications.