Anti-Junctional Adhesion Molecule C antibody [19 H36] (ab16889)

Overview

  • Product nameAnti-Junctional Adhesion Molecule C antibody [19 H36]
    See all Junctional Adhesion Molecule C primary antibodies
  • Description
    Rat monoclonal [19 H36] to Junctional Adhesion Molecule C
  • Tested applicationsSuitable for: IP, Flow Cyt, IHC-Frmore details
    Unsuitable for: IHC-P or WB
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    This information is considered to be commercially sensitive.

  • Positive control
    • High endothelial venules of lymphoid organs, lymphoendothelial cells and endothelial cells of the kidney

Properties

Applications

Our Abpromise guarantee covers the use of ab16889 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
  • Application notesIs unsuitable for IHC-P or WB.
  • Target

    • FunctionParticipates in cell-cell adhesion. It is a counterreceptor for ITGAM, mediating leukocyte-platelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN). The soluble form is a mediator of angiogenesis.
    • Tissue specificityHighest expression in placenta, brain and kidney. Significant expression is detected on platelets. Expressed in intestinal mucosa cells. Expressed in the vascular endothelium. Found in serum (at protein level). Also detected in the synovial fluid of patients with rheumatoid arthritis, psoriatic arthritis or ostearthritis (at protein level).
    • Involvement in diseaseDefects in JAM3 are the cause of hemorrhagic destruction of the brain with subependymal calcification and cataracts (HDBSCC) [MIM:613730]. A syndrome characterized by congenital cataracts and severe brain abnormalities apparently resulting from hemorrhagic destruction of the brain tissue, including the cerebral white matter and basal ganglia. Patients manifest profound developmental delay, and other neurologic features included seizures, spasticity, and hyperreflexia. Brain imaging shows multifocal intraparenchymal hemorrhage with associated liquefaction and massive cystic degeneration, and calcification in the subependymal region and in brain tissue.
    • Sequence similaritiesBelongs to the immunoglobulin superfamily.
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      modifications
      Proteolytically cleaved from endothelial cells surface into a soluble form by ADAM10 and ADAM17; the release of soluble JAM3 is increased by proinflammatory factors.
    • Cellular localizationCell membrane. Cell junction > desmosome. Secreted > extracellular space. In epithelial cells, it is expressed at desmosomes but not at tight junctions. Localizes at the cell surface of endothelial cells; treatment of endothelial cells with vascular endothelial growth factor stimulates recruitement of JAM3 to cell-cell contacts.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ14529 antibody
      • JAM 2 antibody
      • JAM 3 antibody
      • JAM C antibody
      • JAM-2 antibody
      • JAM-3 antibody
      • JAM-C antibody
      • JAM2 antibody
      • Jam3 antibody
      • JAM3_HUMAN antibody
      • JAMC antibody
      • Junctional adhesion molecule 3 antibody
      • Junctional adhesion molecule 3 precursor antibody
      • Junctional adhesion molecule C antibody
      see all

    References for Anti-Junctional Adhesion Molecule C antibody [19 H36] (ab16889)

    ab16889 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you once again for your recent enquiries. I have investigated this case and have obtained the following information for you regarding out JAMC and JAM 2 antibodies. I can understand the confusion, and hope this will help. The SwissProt/Unipro...

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    I'm sorry you have been experiencing a problem with ab16889. As positive control for staining JAM-2 on frozen sections murine tissue of high endothelial venules of lymphoid organs can be used. Or lymphoendothelial cells and the endothelial cells of ...

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