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Product name
Anti-Junctional Adhesion Molecule C antibody [CRAM-18 F26]
See all Junctional Adhesion Molecule C products (5) ...
Description
Rat monoclonal [CRAM-18 F26] to Junctional Adhesion Molecule C
Tested applications
ICC/IF, IHC-Fr, Flow Cytmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Recombinant soluble full length protein (Mouse)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Primary antibody notes
Clone CRAM-18 F26 has been reported to inhibit transendothelial migration (Johnson-Léger, C.A. et al., 2002).
Clonality
Monoclonal
Clone number
CRAM-18 F26
Myeloma
Sp2/0
Isotype
IgG2a
Applications
Show applications key
Our Abpromise guarantee covers the use of ab22539 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
Flow Cyt: 1/10 - 1/25. Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Target
Function
Participates in cell-cell adhesion. It is a counterreceptor for ITGAM, mediating leukocyte-platelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN). The soluble form is a mediator of angiogenesis.
Tissue specificity
Highest expression in placenta, brain and kidney. Significant expression is detected on platelets. Expressed in intestinal mucosa cells. Expressed in the vascular endothelium. Found in serum (at protein level). Also detected in the synovial fluid of patients with rheumatoid arthritis, psoriatic arthritis or ostearthritis (at protein level).
Involvement in disease
Defects in JAM3 are the cause of hemorrhagic destruction of the brain with subependymal calcification and cataracts (HDBSCC) [MIM:613730]. A syndrome characterized by congenital cataracts and severe brain abnormalities apparently resulting from hemorrhagic destruction of the brain tissue, including the cerebral white matter and basal ganglia. Patients manifest profound developmental delay, and other neurologic features included seizures, spasticity, and hyperreflexia. Brain imaging shows multifocal intraparenchymal hemorrhage with associated liquefaction and massive cystic degeneration, and calcification in the subependymal region and in brain tissue.
Sequence similarities
Belongs to the immunoglobulin superfamily.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Post-translational
modifications
Proteolytically cleaved from endothelial cells surface into a soluble form by ADAM10 and ADAM17; the release of soluble JAM3 is increased by proinflammatory factors.
Cellular localization
Cell membrane. Cell junction > desmosome. Secreted > extracellular space. In epithelial cells, it is expressed at desmosomes but not at tight junctions. Localizes at the cell surface of endothelial cells; treatment of endothelial cells with vascular endothelial growth factor stimulates recruitement of JAM3 to cell-cell contacts.
Target information above from: UniProt accessionQ9BX67
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CAM antibody
- FLJ14529 antibody
- JAM 2 antibody
see all
Database links
- Entrez Gene: 83700 Human
- Entrez Gene: 83964 Mouse
- Omim: 606871 Human
- SwissProt: B3KWG9 Human
- SwissProt: Q9BX67 Human
- SwissProt: Q9D8B7 Mouse
- Unigene: 150718 Human
- Unigene: 728339 Human
see all
References for Anti-Junctional Adhesion Molecule C antibody [CRAM-18 F26] (ab22539)
This product has been referenced in:
- Langer HF et al. A novel function of junctional adhesion molecule-C in mediating melanoma cell metastasis. Cancer Res 71:4096-105 (2011). Flow Cyt; Mouse.Read more (PubMed: 21593193) »
- Tormin A et al. Characterization of bone marrow-derived mesenchymal stromal cells (MSC) based on gene expression profiling of functionally defined MSC subsets. Cytotherapy 11:114-28 (2009). Flow Cyt; Human.Read more (PubMed: 19242838) »
See all 3 publications for this product
Publishing research using ab22539? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"