Overview

  • Product name
    Anti-Junctophilin-2 antibody
    See all Junctophilin-2 primary antibodies
  • Description
    Rabbit polyclonal to Junctophilin-2
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic 17 amino acid peptide near the carboxy terminus of human Junctophilin-2 (NP_065166)

  • Positive control
    • Mouse brain tissue lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Affinity chromatography purified via peptide column
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab79071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 74 kDa.Can be blocked with Junctophilin-2 peptide (ab110064).
ELISA Use at an assay dependent dilution.

Target

  • Function
    Contributes to the stabilization of the junctional membrane complexes, which are common to excitable cells and mediate cross-talk between cell surface and intracellular ion channels. Probably acts by anchoring the plasma membrane and endoplasmic/sarcoplasmic reticulum. Contributes to the construction of skeletal muscle triad junctions, and plays an essential role in heart development.
  • Tissue specificity
    Specifically expressed in skeletal muscle and heart.
  • Involvement in disease
    Defects in JPH2 are the cause of cardiomyopathy familial hypertrophic type 17 (CMH17) [MIM:613873]. CMH17 is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
  • Sequence similarities
    Belongs to the junctophilin family.
    Contains 8 MORN repeats.
  • Domain
    The MORN (membrane occupation and recognition nexus) repeats contribute to the plasma membrane binding, possibly by interacting with phospholipids.
  • Cellular localization
    Cell membrane. Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane. Localized predominantly on the plasma membrane. The transmembrane domain is anchored in endoplasmic/sarcoplasmic reticulum membrane, while the N-terminal part associates with the plasma membrane. In heart cells, it predominantly associates along Z lines within myocytes. In skeletal muscle, it is specifically localized at the junction of A and I bands.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ40969 antibody
    • JP-2 antibody
    • JP2 antibody
    • Jph2 antibody
    • JPH2_HUMAN antibody
    • Junctophilin 2 antibody
    • Junctophilin type 2 antibody
    • Junctophilin-2 antibody
    • OTTHUMP00000031651 antibody
    • OTTHUMP00000031652 antibody
    see all

Anti-Junctophilin-2 antibody images

  • Lane 1 : Anti-Junctophilin-2 antibody (ab79071) at 1 µg/ml
    Lane 2 : Anti-Junctophilin-2 antibody (ab79071) at 2 µg/ml

    Lane 1 : mouse brain tissue lysate
    Lane 2 : mouse brain tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 74 kDa
    Observed band size : 74 kDa
    Additional bands at : 64 kDa (possible isoform).

References for Anti-Junctophilin-2 antibody (ab79071)

This product has been referenced in:
  • Bennett HJ  et al. Human junctophilin-2 undergoes a structural rearrangement upon binding PtdIns(3,4,5)P3 and the S101R mutation identified in hypertrophic cardiomyopathy obviates this response. Biochem J 456:205-17 (2013). Read more (PubMed: 24001019) »

See 1 Publication for this product

Product Wall

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (8%)
Sample
Sheep Tissue lysate - whole (Cardiac)
Specification
Cardiac
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Verified customer

Submitted Nov 15 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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