Overview

  • Product nameAnti-JunD antibody
    See all JunD primary antibodies
  • Description
    Rabbit polyclonal to JunD
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Dog, Chimpanzee
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human JunD.

    (Peptide available as ab74427.)

  • Positive control
    • This antibody gave a positive signal in the following Human Nuclear Lysates: HeLa, Jurkat

Properties

Applications

Our Abpromise guarantee covers the use of ab74428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 35 kDa).

Target

  • FunctionTranscription factor binding AP-1 sites.
  • Sequence similaritiesBelongs to the bZIP family. Jun subfamily.
    Contains 1 bZIP domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activator protein 1 antibody
    • AP 1 antibody
    • AP1 antibody
    • Jun D antibody
    • jun D proto oncogene antibody
    • Jund antibody
    • JunD FL isoform antibody
    • JUND_HUMAN antibody
    • Transcription factor jun D antibody
    • Transcription factor jun-D antibody
    see all

Anti-JunD antibody images

  • All lanes : Anti-JunD antibody (ab74428) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 2 : Jurkat nuclear extract lysate (ab14844)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 35 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

References for Anti-JunD antibody (ab74428)

ab74428 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"