Recombinant Anti-JunD antibody [EPR6520] (ab134067)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6520] to JunD
- Suitable for: WB, IP, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-JunD antibody [EPR6520]
See all JunD primary antibodies -
Description
Rabbit monoclonal [EPR6520] to JunD -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IFmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human JunD aa 100-200. The exact sequence is proprietary.
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Positive control
- 293T, Jurkat, HepG2 and HeLa whole cell lysate (ab150035); Human skin and kidney tissue; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.60 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6520 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134067 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000. Detects a band of approximately 39, 43 kDa (predicted molecular weight: 35 kDa).
For unpurified, use 1/5000. |
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IP | (1) |
1/50.
For unpurified, use 1/30. |
ICC/IF | (1) |
1/300.
For unpurified, use 1/200. |
Notes |
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WB
1/10000. Detects a band of approximately 39, 43 kDa (predicted molecular weight: 35 kDa). For unpurified, use 1/5000. |
IP
1/50. For unpurified, use 1/30. |
ICC/IF
1/300. For unpurified, use 1/200. |
Target
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Function
Transcription factor binding AP-1 sites. -
Sequence similarities
Belongs to the bZIP family. Jun subfamily.
Contains 1 bZIP domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3727 Human
- Omim: 165162 Human
- SwissProt: P17535 Human
- Unigene: 2780 Human
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Alternative names
- Activator protein 1 antibody
- AP 1 antibody
- AP1 antibody
see all
Images
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Anti-JunD antibody [EPR6520] (ab134067) at 1/10000 dilution (purified) + HeLa cell lysate at 10 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 35 kDa
Observed band size: 39,43 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Immunofluorescence staining of HeLa cells with unpurified ab134067 at a working dilution of 1 in 300, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 555 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA. The negative control is shown in bottom right hand panel - for the negative control, purified ab134067 was used at a dilution of 1/200 followed by an Alexa Fluor® 488 goat anti-mouse antibody at a dilution of 1/500.
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ab134067 (purified) at 1/50 immunoprecipitating JunD in HepG2 cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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All lanes : Anti-JunD antibody [EPR6520] (ab134067) at 1/10000 dilution (unpurified)
Lane 1 : 293T cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 35 kDa
Observed band size: 39,43 kDa why is the actual band size different from the predicted? -
Immunofluorescence staining of HeLa cells with unpurified ab134067 at a working dilution of 1 in 200, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 555 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA. The negative control is shown in bottom right hand panel - for the negative control, unpurified ab134067 was used at a dilution of 1/200 followed by an Alexa Fluor® 488 goat anti-mouse antibody at a dilution of 1/500.
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Immunofluorescent analysis of HeLa cells labelling JunD with unpurified ab134067 at 1/100 dilution.
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ab134067 (unpurified) at 1/30 immunoprecipitating JunD in HepG2 cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab134067 has been referenced in 5 publications.
- Mao L et al. 5-HT2B-mediated serotonin activation in enterocytes suppresses colitis-associated cancer initiation and promotes cancer progression. Theranostics 12:3928-3945 (2022). PubMed: 35664068
- Breau M et al. The antioxidant N-acetylcysteine protects from lung emphysema but induces lung adenocarcinoma in mice. JCI Insight 4:N/A (2019). PubMed: 31578304
- Ribeiro EA et al. Transcriptional and physiological adaptations in nucleus accumbens somatostatin interneurons that regulate behavioral responses to cocaine. Nat Commun 9:3149 (2018). PubMed: 30089879
- Bigot P et al. Functional characterization of the 12p12.1 renal cancer-susceptibility locus implicates BHLHE41. Nat Commun 7:12098 (2016). EMSA . PubMed: 27384883
- Chen H et al. S100A14: novel modulator of terminal differentiation in esophageal cancer. Mol Cancer Res 11:1542-53 (2013). WB ; Human . PubMed: 24107296