Jurkat cytoplasmic extract lysate (ab14842)

Overview

  • Product nameJurkat cytoplasmic extract lysate
    See all Jurkat lysates
  • General notesCell line: Jurkat (Human T cell lymphoblast-like).
    We recommend aliquoting the extracts into single use fractions and then storing them.
  • Tested applicationsEMSA, WBmore details

Properties

  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
  • Concentration information loading...
  • Research areas
  • BackgroundOften this cell line is called 'JM' (Jurkat and JM are derived from the same patient and are sister clones), occasionally JM may be a subclone with somewhat divergent features. Jurkat cells can be transfected and are therefore useful for studies of blood proto-oncogenes expression, apoptosis and cell survival (e.g HIV). They produce IL-2 and can also be used for studies of differentiation.

Applications

Our Abpromise guarantee covers the use of ab14842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
EMSA Use at an assay dependent dilution.
WB Use at an assay dependent dilution.

References for Jurkat cytoplasmic extract lysate (ab14842)

ab14842 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. We didn't assay our Jurkat cell line for IL-2 production unfortunately, I'm sorry for the inconvenience. Best wishes for 2005,

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"