Jurkat nuclear extract lysate (heat shock treated) (ab14846)

Overview

  • Product nameJurkat nuclear extract lysate (heat shock treated)
    See all Jurkat lysates
  • Description
    Jurkat nuclear extract lysate
  • General notesCell line: Jurkat (Human T cell lymphoblast-like).
    Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).

Properties

  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
  • Concentration information loading...
  • Purification notesThe nuclear extract was prepared from the heat shock-treated Jurkat human T cell leukemia cell line (clone E6-1). Cells were cultured in medium heated at 45°C for 45 minutes immediately prior to harvesting.
  • Research areas
  • BackgroundOften this cell line is called 'JM' (Jurkat and JM are derived from the same patient and are sister clones), occasionally JM may be a subclone with somewhat divergent features. Jurkat cells can be transfected and are therefore useful for studies of blood proto-oncogenes expression, apoptosis and cell survival (e.g HIV). They produce IL-2 and can also be used for studies of differentiation.

References for Jurkat nuclear extract lysate (heat shock treated) (ab14846)

ab14846 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"