General notesCell line: Jurkat (Human T cell lymphoblast-like).
Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
Cells were cultured in medium supplemented with 50 ng/mL TPA (phorbol, 12-myristate, 13 acetate) and 0.5 µM calcium ionophore A23187 (CI) for two hours at 37°C immediately prior to harvesting.
Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
BackgroundOften this cell line is called 'JM' (Jurkat and JM are derived from the same patient and are sister clones), occasionally JM may be a subclone with somewhat divergent features. Jurkat cells can be transfected and are therefore useful for studies of blood proto-oncogenes expression, apoptosis and cell survival (e.g HIV). They produce IL-2 and can also be used for studies of differentiation.
References for Jurkat nuclear extract lysate (TPA + CI stimulated) (ab14850)
has not yet been referenced specifically in any publications.
Publishing research using ab14850? Please let us know so that we can cite the reference in this datasheet.
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