Jurkat nuclear extract lysate (TPA + CI stimulated) (ab14850)

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Overview

  • Product name
    Jurkat nuclear extract lysate (TPA + CI stimulated)
    See all Jurkat lysates
  • Description
    Jurkat nuclear extract lysate
  • General notes
    Cell line: Jurkat (Human T cell lymphoblast-like).
    Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
    Cells were cultured in medium supplemented with 50 ng/mL TPA (phorbol, 12-myristate, 13 acetate) and 0.5 µM calcium ionophore A23187 (CI) for two hours at 37°C immediately prior to harvesting.

Properties

  • Form
    Liquid
  • Storage instructions
    Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
  • Concentration information loading...
  • Research areas
  • Background
    Often this cell line is called 'JM' (Jurkat and JM are derived from the same patient and are sister clones), occasionally JM may be a subclone with somewhat divergent features. Jurkat cells can be transfected and are therefore useful for studies of blood proto-oncogenes expression, apoptosis and cell survival (e.g HIV). They produce IL-2 and can also be used for studies of differentiation.

References for Jurkat nuclear extract lysate (TPA + CI stimulated) (ab14850)

ab14850 has not yet been referenced specifically in any publications.

Publishing research using ab14850? Please let us know so that we can cite the reference in this datasheet.

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1 Q&A

10
Votes: Highest First

Ich habe den WB-Fragebogen ausgefuellt, ich hoffe Sie koennen mir helfen.

Vielen Dank für Ihre Anfrage und dafür, dass Sie sich die Zeit genommen haben, unseren Fragebogen auszufüllen.

Die Bande bei 50 kDa sieht in der Tat unspezifisch aus; dies wäre allerdings ja nicht weiter schlimm, wenn Sie die spezifische Ban...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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