Overview

  • Product nameAnti-K Cadherin antibody
    See all K Cadherin primary antibodies
  • Description
    Rabbit polyclonal to K Cadherin
  • Tested applicationsSuitable for: IHC-P, Flow Cyt, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A KLH conjugated synthetic peptide selected from the C-terminal region of human K Cadherin.

  • Positive control
    • 293 cell line lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab64917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50.
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/50 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 88 kDa).

In recent lot testing (Dec 2013), the apparent MW (130 kDa) is higher than the predicted MW (88 kDa) in over expression lysate, human brain and U251 cells under reduced and denatured conditions.

ELISA 1/1000.

Target

  • FunctionCadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.
  • Tissue specificityHighly expressed in brain, cerebellum, and kidney. Lung, pancreas, and gastric mucosa show a weak expression. Also expressed in certain liver and kidney carcinomas.
  • Sequence similaritiesContains 5 cadherin domains.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAD6 antibody
    • CADH6_HUMAN antibody
    • Cadherin 6 antibody
    • Cadherin 6 type 2 antibody
    • Cadherin 6 type 2 K cadherin (fetal kidney) antibody
    • Cadherin fetal kidney antibody
    • Cadherin-6 antibody
    • Cadherin6 antibody
    • CDH 6 antibody
    • Cdh6 antibody
    • K-cadherin antibody
    • KCAD antibody
    • Kidney cadherin antibody
    see all

Anti-K Cadherin antibody images

  • Flow cytometry analysis of NCI-H292 cells labeling K Cadherin with ab64917. A FITC-conjugated Goat anti-rabbit was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human hepatocarcinoma tissue sections labeling K Cadherin with ab64917.

  • Anti-K Cadherin antibody (ab64917) at 1/60 dilution + 293 cell line lysates at 35 µg

    Predicted band size : 88 kDa
    Observed band size : 88 kDa
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.

References for Anti-K Cadherin antibody (ab64917)

This product has been referenced in:
  • Schiffmacher AT  et al. Cadherin-6B is proteolytically processed during epithelial-to-mesenchymal transitions of the cranial neural crest. Mol Biol Cell 25:41-54 (2014). WB . Read more (PubMed: 24196837) »

See 1 Publication for this product

Product Wall

Human K Cadherin full length protein (ab132981) refers to the isoform 2 of the protein (Uniprot ID P55285) which is composed of 633 amino acids. This isoform lacks amino acids 664-790 that can be found on the isoform 1.


Anti-K Cadherin ...

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The antibody is indeed ab64917 which is now added in waiting for stock order 1184710.

Please let me know if you have any other question

Have a nice day!

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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My colleague is out of office today I am sorry to hear that your customer is experiencing difficulties using the antibody.

The batch/lot has been used in a publication with IF data:


Montserrat N et al. Generation of induced p...

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Thank you for contacting us.

The immunogen peptide islocated between aa.

As you seem to be quite happy about the antibody's performance in WB and hopefully soon in ELISA, maybe I could encourage you to write an Abreview to share yo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"