Overview

  • Product name
  • Description
    Rabbit polyclonal to K0776
  • Tested applications
    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 175-225 of Human K0776 using the numbering given in entry NP_056138.1.

  • Positive control
    • HeLa whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab123500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      E3 UFM1-protein ligase that mediates ufmylation of target proteins such as DDRGK1/C20orf116. The function of ufmylation is unknown. May act as a tumor suppressor by inhibiting cell invasion, blocking NF-kappa-B signaling and increasing stability of CDK5RAP3.
    • Tissue specificity
      Ubiquitously expressed, with a high expression in liver (at protein level). Low expression in several invasive hepatocellular carcinomas, such HepG2, Hep3B, HLE and PLC.
    • Sequence similarities
      Belongs to the UFL1 family.
    • Post-translational
      modifications
      Ubiquitinated, leading to its degradation by the proteasome. Interaction with CDK5RAP3 protects each other against ubiquitination and degradation via the proteasome.
    • Cellular localization
      Endoplasmic reticulum. Cytoplasm. In cytoplasm colocalizes with CDK5RAP3.
    • Information by UniProt
    • Database links
    • Alternative names
      • E3 UFM1-protein ligase 1 antibody
      • KIAA0776 antibody
      • LZAP-binding protein antibody
      • Maxer antibody
      • NLBP antibody
      • RCAD antibody
      • UFL1 antibody
      • UFL1_HUMAN antibody
      • UFM1 specific ligase 1 antibody
      see all

    Images

    • Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab123500 for IP at 6 µg/mg lysate.
      Subsequent Western blot detection used an antibody which recognized a downstream epitope of K0776 at 0.4 µg/ml.
      Detection: Chemiluminescence with an exposure time of 10 seconds.

    References

    ab123500 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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