K562 whole cell lysate (ab29306)

Overview

  • Product nameK562 whole cell lysate
    See all K562 lysates
  • General notesCell line: K562 (Human erythromyeloblastoid leukemia).


    Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC.

  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundK-562 is an erythroleukemia cell line derived from a chronic myeloid leukemia patient in blast crisis. Recent studies have shown the K562 blasts are multipotential, hematopoietic malignant cells that spontaneously differentiate into recognisable progenitors of the erythrocyte, granulocyte and monocytic series. Cells are also used for haemoglobin synthesis, antitumour testing, tumorigenicity, differentiation, pharmacodynamics, cloning, cytotoxicity, cell biology, cellular effects of hyperthermia, and natural killer assays.

Applications

Our Abpromise guarantee covers the use of ab29306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.

References for K562 whole cell lysate (ab29306)

ab29306 has not yet been referenced specifically in any publications.

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I suggest increasing the primary antibody concentration based on the images which show poor bands for the expected size (˜95kDa)as compared to the 70kDa non specific band. I also suggest using BSA as a blocking...

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The lysates ab29306 and ab7911 were prepared using slightly different techniques, but will give equivalent results in WB. Either of these lysates will be guaranteed for use as a positive control with ab93373. We r...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"