Anti-KA1 antibody (ab67402)

Overview

  • Product nameAnti-KA1 antibody
    See all KA1 primary antibodies
  • Description
    Rabbit polyclonal to KA1
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human KA1 aa 1-18 (C terminal). An 18 amino acid synthetic peptide near the carboxy terminus of the mouse Grik4.
    (Peptide available as ab209614)

  • Positive control
    • Rat brain lysate and human brain tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Allow to warm to room temp and agitate gently before aliquotting. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab67402 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
IHC-P
WB
  • Application notesIHC-P: Use at a concentration of 2.5 µg/ml.
    WB: Use at a concentration of 0.5 - 2 µg/ml. Detects a band of approximately <97 kDa (predicted molecular weight: 108 kDa).

    N.B. KA1 often migrates at a lower molecular weight in SDS-PAGE.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionReceptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists.
    • Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK4 subfamily.
    • Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • EAA 1 antibody
      • EAA1 antibody
      • Excitatory amino acid receptor 1 antibody
      • GluK4 antibody
      • Glutamate receptor antibody
      • Glutamate receptor ionotropic kainate 4 antibody
      • Glutamate receptor ionotropic kainate 4 precursor antibody
      • Glutamate receptor KA 1 antibody
      • Glutamate receptor KA 1precursor antibody
      • Glutamate receptor KA-1 antibody
      • Glutamate receptor KA1 antibody
      • Glutamate receptor, ionotropic kainate 4 antibody
      • GRIK 4 antibody
      • GRIK antibody
      • Grik4 antibody
      • GRIK4_HUMAN antibody
      • ionotropic kainate 4 antibody
      • KA 1 antibody
      • KA1 antibody
      • OTTHUMP00000045951 antibody
      • OTTHUMP00000231881 antibody
      see all

    Anti-KA1 antibody images

    • Lane 1 : Anti-KA1 antibody (ab67402) at 0.5 µg/ml
      Lane 2 : Anti-KA1 antibody (ab67402) at 1 µg/ml
      Lane 3 : Anti-KA1 antibody (ab67402) at 2 µg/ml

      Lane 1 : Rat brain lysate
      Lane 2 : Rat brain lysate
      Lane 3 : Rat brain lysate

      Lysates/proteins at 15 µg per lane.


      Predicted band size : 108 kDa
      Observed band size : <97 kDa (why is the actual band size different from the predicted?)
    • ab67402 at 2.5µg/ml staining of KA1 in human brain tissue by IHC-P.
    • Immunofluorescence of Grik4 in Human Brain cells using ab67402 at 20 ug/ml.

    References for Anti-KA1 antibody (ab67402)

    ab67402 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 25 µg
    Gel Running Conditions Reduced Denaturing (10% gel)
    Sample Mouse Tissue lysate - whole (Brain)
    Specification Brain
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 16°C
    Username

    Dr. Garry Whitehead

    Verified customer

    Submitted Sep 23 2013

    The exact immunogen sequence for ab67402 is proprietary, but I can confirm that it is from within the last 50 amino acids of the human KA1 protein.
    Unfortunately, this antibody currently has not been used in any published papers.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"