Overview

  • Product nameAnti-KA1 antibody
    See all KA1 primary antibodies
  • Description
    Rabbit polyclonal to KA1
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 14 amino acid peptide near the amino terminus of human KA1.

  • Positive control
    • Rat brain lysate and human brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab67404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately <97 kDa (predicted molecular weight: 107 kDa).
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionReceptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists.
  • Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK4 subfamily.
  • Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EAA 1 antibody
    • EAA1 antibody
    • Excitatory amino acid receptor 1 antibody
    • GluK4 antibody
    • Glutamate receptor antibody
    • Glutamate receptor ionotropic kainate 4 antibody
    • Glutamate receptor ionotropic kainate 4 precursor antibody
    • Glutamate receptor KA 1 antibody
    • Glutamate receptor KA 1precursor antibody
    • Glutamate receptor KA-1 antibody
    • Glutamate receptor KA1 antibody
    • Glutamate receptor, ionotropic kainate 4 antibody
    • GRIK 4 antibody
    • GRIK antibody
    • Grik4 antibody
    • GRIK4_HUMAN antibody
    • ionotropic kainate 4 antibody
    • KA 1 antibody
    • KA1 antibody
    • OTTHUMP00000045951 antibody
    • OTTHUMP00000231881 antibody
    see all

Anti-KA1 antibody images

  • Lane 1 : Anti-KA1 antibody (ab67404) at 0.5 µg/ml
    Lane 2 : Anti-KA1 antibody (ab67404) at 1 µg/ml
    Lane 3 : Anti-KA1 antibody (ab67404) at 2 µg/ml

    Lane 1 : rat brain lysate
    Lane 2 : rat brain lysate
    Lane 3 : rat brain lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 107 kDa
    Observed band size : <97 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 108 kDa. We are unsure as to the identity of these extra bands.
  • ab67404 at 2.5µg/ml staining of KA1 in human brain tissue by IHC-P.
  • ICC/IF image of ab67404 stained SKNSH cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab67404, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Immunofluorescence of Grik4 in Human Brain cells using ab67404 at 20 ug/ml.

References for Anti-KA1 antibody (ab67404)

This product has been referenced in:
  • Marrocco J  et al. Anxiety-like behavior of prenatally stressed rats is associated with a selective reduction of glutamate release in the ventral hippocampus. J Neurosci 32:17143-54 (2012). Rat . Read more (PubMed: 23197707) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"