The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesGSA: Use at an assay dependent dilution. Supershifts a specific methyl-CpG complex.
ICC: Use at a concentration of 1 µg/ml for positive nuclear staining in NIH3T3 cells fixed with 100% methanol.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 0.5 - 2 µg/ml on RIPA lysates from 3T3/A31 cells (ECL). Detects a band of approximately 97 kDa (predicted molecular weight: 75 kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionTranscriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3'. Recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. May contribute to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2.
Tissue specificityExpressed in vascular endothelium.