Anti-Kaiso antibody [6F / 6F8] - ChIP Grade (ab12723)

Overview

  • Product name
    Anti-Kaiso antibody [6F / 6F8] - ChIP Grade
    See all Kaiso primary antibodies
  • Description
    Mouse monoclonal [6F / 6F8] to Kaiso - ChIP Grade
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, ChIP, ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Hamster, Dog, Human
  • Immunogen

    His-tagged Kaiso fusion protein (Human) lacking the zinc finger region.

  • Positive control
    • CSML0 cells (IP then Western). In Flow Cytometry, this antibody gave a positive signal in HeLa cells.
  • General notes

    This antibody clone is manufactured by Abcam.

    We can conjugate this antibody to FITC for you (please see ab150256 for details).

Properties

Applications

Our Abpromise guarantee covers the use of ab12723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 80 kDa).
IP Use at an assay dependent concentration.

Target

  • Function
    Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3'. Recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. May contribute to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2.
  • Tissue specificity
    Expressed in vascular endothelium.
  • Sequence similarities
    Contains 1 BTB (POZ) domain.
    Contains 3 C2H2-type zinc fingers.
  • Cellular localization
    Nucleus. Cytoplasm. Also cytoplasmic in cells grown at high densities.
  • Information by UniProt
  • Database links
  • Alternative names
    • Kaiso antibody
    • Kaiso protein antibody
    • Kaiso transcription factor antibody
    • KAISO_HUMAN antibody
    • Transcriptional regulator Kaiso antibody
    • ZBTB 33 antibody
    • Zbtb33 antibody
    • Zinc finger and BTB domain containing 33 antibody
    • Zinc finger and BTB domain-containing protein 33 antibody
    • Zinc finger transcription factor Kaiso antibody
    • ZNF 348 antibody
    • ZNF Kaiso antibody
    • ZNF348 antibody
    see all

Images

  • Immunofluorescence using clone 6F / 6F8 on C2C12 cells.


  • Predicted band size : 80 kDa

    Kaiso was IP'd from CSML0 cells using clone 6F / 6F8 and western blotted using clone 6F / 6F8.

    Kaiso was IP'd from CSML0 cells using clone 6F / 6F8 and western blotted using clone 6F / 6F8.
  • Overlay histogram showing HeLa cells stained with ab12723 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12723, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunofluroesence analysis of Human ARPE-19 cells, staining Kaiso using ab12723.


  • Predicted band size : 80 kDa

    Image from Chen HC et al., PLoS One. 2012;7(5):e36864. Epub 2012 May 9.; Fig 4.; doi:10.1371/journal.pone.0036864; May 9, 2012, PLoS ONE 7(5): e36864.

    Representative western blot detecting Kaiso in ARPE-19 cells using ab12723.

References

This product has been referenced in:
  • Xue X  et al. Kaiso protects human umbilical vein endothelial cells against apoptosis by differentially regulating the expression of B-cell CLL/lymphoma 2 family members. Sci Rep 7:7116 (2017). WB, ChIP, EMSA, ICC/IF ; Human . Read more (PubMed: 28769046) »
  • Zhao C  et al. Dual regulatory switch through interactions of Tcf7l2/Tcf4 with stage-specific partners propels oligodendroglial maturation. Nat Commun 7:10883 (2016). IHC, ChIP . Read more (PubMed: 26955760) »

See all 13 Publications for this product

Customer reviews and Q&As


It seems that this antibody is sometimes referred to as 6F8 and sometimes as 6F. So we've labeled it with both clone names.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Sample
Human Cell (SW480)
Specification
SW480
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 11 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Sample
Mouse Cell (NIH 3T3)
Specification
NIH 3T3
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 11 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Human Cell lysate - whole cell (sw480)
Specification
sw480
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Sep 11 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mice APC Min)
Specification
Mice APC Min
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris buffer
Permeabilization
No
Blocking step
1% gelatin 10%FBS as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Mr. Raul Peña

Verified customer

Submitted Dec 18 2012

Thank you for contacting Abcam and for letting us know that ab12723 was used in that publication. I have passed this information to our references team, who will review the article and then upload that reference to our website.
If there is anythin...

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Thank you for your inquiry. I heard back from our lab that unfortunately we do not have any more information on the immunogen sequence other than what is stated on the datasheet.    Ideally,  for an antibody that works in ChIP, the immunogen se...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Pancreas)
Specification
Pancreas
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization
No
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 45 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Apr 06 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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