Anti-Kaiso antibody [6F / 6F8] (ab12723)
Key features and details
- Mouse monoclonal [6F / 6F8] to Kaiso
- Suitable for: ICC/IF, WB, Flow Cyt (Intra)
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-Kaiso antibody [6F / 6F8]
See all Kaiso primary antibodies -
Description
Mouse monoclonal [6F / 6F8] to Kaiso -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WB, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Hamster, Dog -
Immunogen
Fusion protein corresponding to Human Kaiso.
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Positive control
- CSML0 cells (IP then Western). Flow Cyt (Intra): HeLa cells.
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General notes
We can conjugate this antibody to FITC for you (please see ab150256 for details).
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Contains 0.4M Arginine -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
6F / 6F8 -
Isotype
IgG1 -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab12723 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (2) |
Use at an assay dependent concentration.
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WB | (1) |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 80 kDa).
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Flow Cyt (Intra) |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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ICC/IF
Use at an assay dependent concentration. |
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 80 kDa). |
Flow Cyt (Intra)
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3'. Recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. May contribute to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2. -
Tissue specificity
Expressed in vascular endothelium. -
Sequence similarities
Contains 1 BTB (POZ) domain.
Contains 3 C2H2-type zinc fingers. -
Cellular localization
Nucleus. Cytoplasm. Also cytoplasmic in cells grown at high densities. - Information by UniProt
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Database links
- Entrez Gene: 10009 Human
- Entrez Gene: 56805 Mouse
- Entrez Gene: 501506 Rat
- Omim: 300329 Human
- SwissProt: Q86T24 Human
- SwissProt: Q8BN78 Mouse
- Unigene: 143604 Human
- Unigene: 391248 Mouse
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Alternative names
- Kaiso antibody
- Kaiso protein antibody
- Kaiso transcription factor antibody
see all
Images
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Immunofluorescence using clone 6F / 6F8 on C2C12 cells.
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Kaiso was IP'd from CSML0 cells using clone 6F / 6F8 and western blotted using clone 6F / 6F8.
Kaiso was IP'd from CSML0 cells using clone 6F / 6F8 and western blotted using clone 6F / 6F8. -
Overlay histogram showing HeLa cells stained with ab12723 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12723, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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Immunofluroesence analysis of Human ARPE-19 cells, staining Kaiso using ab12723.
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Representative western blot detecting Kaiso in ARPE-19 cells using ab12723.
Datasheets and documents
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SDS download
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Datasheet download
References (19)
ab12723 has been referenced in 19 publications.
- Singhal SK et al. Kaiso (ZBTB33) subcellular partitioning functionally links LC3A/B, the tumor microenvironment, and breast cancer survival. Commun Biol 4:150 (2021). PubMed: 33526872
- Tong W et al. Kaiso regulates osteoblast differentiation and mineralization via the Itga10/PI3K/AKT signaling pathway. Int J Mol Med 47:N/A (2021). PubMed: 33576467
- Hiramatsu S et al. Regulation of Cathepsin E gene expression by the transcription factor Kaiso in MRL/lpr mice derived CD4+ T cells. Sci Rep 9:3054 (2019). PubMed: 30816218
- Choi SH et al. Temporal and differential regulation of KAISO-controlled transcription by phosphorylated and acetylated p53 highlights a crucial regulatory role of apoptosis. J Biol Chem 294:12957-12974 (2019). PubMed: 31296660
- Abisoye-Ogunniyan A et al. Transcriptional repressor Kaiso promotes epithelial to mesenchymal transition and metastasis in prostate cancer through direct regulation of miR-200c. Cancer Lett 431:1-10 (2018). ICC/IF . PubMed: 29751044