The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1 - 1/1000.
Use a concentration of 20 - 40 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionMay be an adhesion-like molecule with anti-protease activity.
Involvement in diseaseDefects in KAL1 are the cause of Kallmann syndrome type 1 (KAL1) [MIM:308700]; also known as hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin-releasing hormone-synthesizing neurons. In some patients other developmental anomalies can be present, which include renal agenesis, cleft lip and/or palate, selective tooth agenesis, and bimanual synkinesis. In some cases anosmia may be absent or inconspicuous.
ab115270, at 40 µg/ml, staining KAL1 in formalin fixed, paraffin embedded Human skeletal muscle tissue by Immunohistochemistry. After incubation with the primary antibody, slides were incubated with biotinylated goat anti-rabbit IgG secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
References for Anti-KAL1 antibody (ab115270)
has not yet been referenced specifically in any publications.
Publishing research using ab115270? Please let us know so that we can cite the reference in this datasheet.
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"