SpecificityMonospecific for tissue kallikrein. Crossreacts with tissue kallikreins from urine, pancreas, saliva, kidney, intestine, seminal plasma and serum. Does not crossreact with plasma kallikrein.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000.
FunctionGlandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
Tissue specificityIsoform 2 is expressed in pancreas, salivary glands, kidney, colon, prostate gland, testis, spleen and the colon adenocarcinoma cell line T84.
Sequence similaritiesBelongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain.
Post-translational modificationsThe O-linked polysaccharides on Ser-93, Ser-104 and Ser-167 are probably the mucin type linked to GalNAc. In PubMed:3163150, GalNAc was detected with the corresponding peptides but not located.