Anti-Kallikrein 1 antibody - Kallikrein loop (ab28289)


  • Product nameAnti-Kallikrein 1 antibody - Kallikrein loop
    See all Kallikrein 1 primary antibodies
  • Description
    Rabbit polyclonal to Kallikrein 1 - Kallikrein loop
  • Specificityab28289 recognises the Kallikrein loop of Kallikrein 1. It does not react with the other human Kallikreins.
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to the the Kallikrein loop area of human Kallikrein 1. (Peptide available as ab41419.)



Our Abpromise guarantee covers the use of ab28289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 28 kDa.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.


  • FunctionGlandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
  • Tissue specificityIsoform 2 is expressed in pancreas, salivary glands, kidney, colon, prostate gland, testis, spleen and the colon adenocarcinoma cell line T84.
  • Sequence similaritiesBelongs to the peptidase S1 family. Kallikrein subfamily.
    Contains 1 peptidase S1 domain.
  • Post-translational
    The O-linked polysaccharides on Ser-93, Ser-104 and Ser-167 are probably the mucin type linked to GalNAc. In PubMed:3163150, GalNAc was detected with the corresponding peptides but not located.
  • Information by UniProt
  • Database links
  • Alternative names
    • Glandular kallikrein 1 antibody
    • hK 1 antibody
    • hK1 antibody
    • Kallikrein serine protease 1 antibody
    • Kallikrein-1 antibody
    • Kidney/pancreas/salivary gland kallikrein antibody
    • Klk 6 antibody
    • KLK1 antibody
    • KLK1_HUMAN antibody
    • Klk6 antibody
    • KLKR antibody
    • Tissue kallikrein antibody
    see all

Anti-Kallikrein 1 antibody - Kallikrein loop images

  • All lanes : Anti-Kallikrein 1 antibody - Kallikrein loop (ab28289) at 1/1000 dilution

    Lane 1 : Kalikrein 1 at 0.05 µg
    Lane 2 : Kalikrein 1 at 0.01 µg
    Lane 3 : Kalikrein 1 at 0.001 µg

    Predicted band size : 28 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • IHC image of ab28289 staining in human normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab28289, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Kallikrein 1 antibody - Kallikrein loop (ab28289)

This product has been referenced in:
  • Tang SC  et al. Bradykinin and high glucose promote renal tubular inflammation. Nephrol Dial Transplant 25:698-710 (2010). ICC/IF ; Human . Read more (PubMed: 19923143) »
  • Cassim B  et al. Kallikreins, kininogens and kinin receptors on circulating and synovial fluid neutrophils: role in kinin generation in rheumatoid arthritis. Rheumatology (Oxford) 48:490-6 (2009). ICC/IF, IHC ; Human . Read more (PubMed: 19254919) »

See all 2 Publications for this product

Product Wall

Following up my previous message, immunogens for the kallikrein antibodies ab28289 and ab40497 were aligned with the mouse canonical sequence ( The ab28289 epitope falls in a region of relatively low seq...

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Thank you for contacting us. I am still waiting for our source for the kallikrein antibodies ab28289 and ab40497 to send their alignments of the immunogens and mouse kallikrein but I have checked the homology of the immunogens for the other four ant...

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