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Products:Cardiovascular >> Blood >> Serum Proteins
Overview
Product name
Anti-Kallikrein 1 antibody - Kallikrein loop
See all Kallikrein 1 products (5) ...
Description
Rabbit polyclonal to Kallikrein 1 - Kallikrein loop
Specificity
ab28289 recognises the Kallikrein loop of Kallikrein 1. It does not react with the other human Kallikreins.
Tested applications
WB, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide corresponding to the the Kallikrein loop area of human Kallikrein 1. (Peptide available as ab41419.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Kallikreins
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Kallikreins
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> Kallikreins
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Kallikreins
Cardiovascular >> Blood >> Coagulation >> Intrinsic
Cardiovascular >> Blood >> Serum Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28289 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/5000.Predicted molecular weight: 28 kDa.
IHC-P: Use a concentration of 1 µg/mlPerform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF: Use at an assay dependent dilution.
Target
Function
Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
Tissue specificity
Isoform 2 is expressed in pancreas, salivary glands, kidney, colon, prostate gland, testis, spleen and the colon adenocarcinoma cell line T84.
Sequence similarities
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain.
Post-translational
modifications
The O-linked polysaccharides on Ser-93, Ser-104 and Ser-167 are probably the mucin type linked to GalNAc. In PubMed:3163150, GalNAc was detected with the corresponding peptides but not located.
Target information above from: UniProt accessionP06870
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- hK1 antibody
- Kidney/pancreas/salivary gland kallikrein antibody
- Glandular kallikrein 1 antibody
see all
Database links
- Entrez Gene: 3816 Human
- Omim: 147910 Human
- SwissProt: P06870 Human
- Unigene: 123107 Human
Anti-Kallikrein 1 antibody - Kallikrein loop images:
Western blot - Kallikrein 1 antibody - Kallikrein loop (ab28289)
All lanes : Anti-Kallikrein 1 antibody - Kallikrein loop (ab28289) at 1/1000 dilution
Lane 1 : Kalikrein 1 at 0.05 µg
Lane 2 : Kalikrein 1 at 0.01 µg
Lane 3 : Kalikrein 1 at 0.001 µg
Predicted band size : 28 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Kallikrein 1 antibody - Kallikrein loop(ab28289)
IHC image of ab28289 staining in human normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab28289, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
References for Anti-Kallikrein 1 antibody - Kallikrein loop (ab28289)
This product has been referenced in:
- Tang SCet al. Bradykinin and high glucose promote renal tubular inflammation. Nephrol Dial Transplant 25:698-710 (2010). ICC/IF; Human.Read more (PubMed: 19923143) »
- Cassim Bet al. Kallikreins, kininogens and kinin receptors on circulating and synovial fluid neutrophils: role in kinin generation in rheumatoid arthritis. Rheumatology (Oxford) 48:490-6 (2009). ICC/IF, IHC; Human.Read more (PubMed: 19254919) »
See all 2 publications for this product
Publishing research using ab28289? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"