Anti-Kallikrein 2/KLK2 antibody (ab232980)
Key features and details
- Rabbit polyclonal to Kallikrein 2/KLK2
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-Kallikrein 2/KLK2 antibody
See all Kallikrein 2/KLK2 primary antibodies -
Description
Rabbit polyclonal to Kallikrein 2/KLK2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant full length protein (His-tag) corresponding to Human Kallikrein 2/KLK2 aa 1 to the C-terminus. Expressed in E. coli. N-terminal tag.
Database link: P20151-1 -
Positive control
- WB: Recombinant human Kallikrein 2/KLK2 protein. Rat prostate gland tissue lysate. IHC-P: Human cerebrum, glioma, prostate, prostate cancer, breast cancer and colorectal cancer tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
-
Purity
Protein A purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab232980 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 5 - 20 µg/ml.
|
|
WB |
Use a concentration of 0.5 - 2 µg/ml.
|
Notes |
---|
IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.5 - 2 µg/ml. |
Target
-
Function
Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. -
Sequence similarities
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain. - Information by UniProt
-
Database links
- Entrez Gene: 3817 Human
- Entrez Gene: 690645 Rat
- Omim: 147960 Human
- SwissProt: P20151 Human
- SwissProt: P00759 Rat
- Unigene: 515560 Human
-
Alternative names
- Glandular kallikrein 2 antibody
- Glandular kallikrein-1 antibody
- hGK 1 antibody
see all
Images
-
Anti-Kallikrein 2/KLK2 antibody (ab232980) at 2 µg/ml + Rat prostate gland tissue lysate
Secondary
HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution -
Formalin-fixed, paraffin-embedded human colorectal cancer tissue stained for Kallikrein 2/KLK2 with ab232980 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
-
Formalin-fixed, paraffin-embedded human breast cancer tissue stained for Kallikrein 2/KLK2 with ab232980 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
-
Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for Kallikrein 2/KLK2 with ab232980 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
-
Formalin-fixed, paraffin-embedded human prostate tissue stained for Kallikrein 2/KLK2 with ab232980 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
-
Formalin-fixed, paraffin-embedded human glioma tissue stained for Kallikrein 2/KLK2 with ab232980 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
-
Formalin-fixed, paraffin-embedded human cerebrum tissue stained for Kallikrein 2/KLK2 with ab232980 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
-
Anti-Kallikrein 2/KLK2 antibody (ab232980) at 2 µg/ml + Recombinant human Kallikrein 2/KLK2 protein
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab232980 has not yet been referenced specifically in any publications.